PMID: 58029May 1, 1976Paper

Generation of cytotoxic lymphocytes in vitro: response of immune rat spleen cells to a syngeneic gross virus-induced lymphoma in mixed lymphocyte-tumor culture

The Journal of Immunology : Official Journal of the American Association of Immunologists
I D BernsteinE F Cohen

Abstract

Spleen cells from W/Fu rats 4 to 6 weeks after immunization with syngeneic Gross virus-induced lymphoma (C58NT)D cells usually lack detectable activity in a short-term 51Cr release assay. The results presented here demonstrate that these spleen cells retain the capacity to generate significant proliferative and cytotoxic activity upon re-exposure to mitomycin C-treated (C58NT)D cells in vitro. Optimal conditions were defined in W/Fu rats for this secondary immune response in vitro to the (C58NT)D cells. The cytotoxic response was observed to be quantitative, reproducible, and specific. Optimal generation occurred 5 days after initiation of cultures with a 30:1 responding cell:stimulating cell ratio. In vitro generated cytotoxic cells inhibit tumor growth in vivo when administered as a mixture with tumor cells.

Related Concepts

Gross Virus
Antigenic Specificity
Neoplastic Cell Transformation
Microcytotoxicity Test
Lymphocyte Culture Test, Mixed
Lymphocyte Activation
Lymphoma
Mitomycins
Rats, Inbred BN
Rats, Inbred WF

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