PMID: 6970341Feb 26, 1981Paper

Generation of cytotoxic T-lymphocyte precursor cells in T-cell colonies grown in vitro

Nature
L M Ching, R G Miller

Abstract

The role of the thymus in T-lymphocyte differentiation remains unclear. The demonstration that the thymus can restrict the T-lymphocyte specificity repertoire suggests that T cells acquire specificity within the thymus. However, the demonstrations of immunocompetent helper T cells and cytotoxic T-lymphocyte precursor cells (CLPs) in athymic nude mice suggest that the acquisition of some T-cell reactivity may occur without the thymus. We have been using T-cell colonies grown in vitro as a model system for studying various aspects of T-cell differentiation in both mouse and man. In one study we showed that CLPs can be found in T-cell colonies grown from spleen cells of normal mice, each colony containing CLPs of several different specificities. The colonies containing CLPs are not clonal, appearing to have a colony-forming unit (CFU-T) of two (perhaps three) cells. Here we provide direct evidence that the CLPs are spontaneously produced in the colonies. In addition, the cells of the CFU-T were characterized with antisera directed against the cell-surface marker Thy-1, which is present on all murine T cells, and the cell-surface markers Lyt-1 and Lyt-2, which are differentially distributed on different T-cell subclasses. We found ...Continue Reading

References

Jan 1, 1978·Immunological Reviews·O Stutman
Jul 14, 1977·Nature·S Gillis, K A Smith
Jun 1, 1979·The Journal of Experimental Medicine·S GillisK A Smith
Mar 1, 1978·The Journal of Experimental Medicine·R M ZinkernagelJ Klein
Sep 1, 1978·The Journal of Experimental Medicine·P J Fink, M J Bevan
Jan 1, 1978·Advances in Cancer Research·R B Herberman, H T Holden
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Mar 10, 1971·Nature: New Biology·C P StannersH Green

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