Abstract
The role of the thymus in T-lymphocyte differentiation remains unclear. The demonstration that the thymus can restrict the T-lymphocyte specificity repertoire suggests that T cells acquire specificity within the thymus. However, the demonstrations of immunocompetent helper T cells and cytotoxic T-lymphocyte precursor cells (CLPs) in athymic nude mice suggest that the acquisition of some T-cell reactivity may occur without the thymus. We have been using T-cell colonies grown in vitro as a model system for studying various aspects of T-cell differentiation in both mouse and man. In one study we showed that CLPs can be found in T-cell colonies grown from spleen cells of normal mice, each colony containing CLPs of several different specificities. The colonies containing CLPs are not clonal, appearing to have a colony-forming unit (CFU-T) of two (perhaps three) cells. Here we provide direct evidence that the CLPs are spontaneously produced in the colonies. In addition, the cells of the CFU-T were characterized with antisera directed against the cell-surface marker Thy-1, which is present on all murine T cells, and the cell-surface markers Lyt-1 and Lyt-2, which are differentially distributed on different T-cell subclasses. We found ...Continue Reading
References
Jan 1, 1978·Immunological Reviews·O Stutman
Mar 1, 1976·European Journal of Immunology·M J BevanM Cohn
Jul 14, 1977·Nature·S Gillis, K A Smith
Jun 1, 1979·The Journal of Experimental Medicine·S GillisK A Smith
Sep 10, 1976·Science·D A MorganR Gallo
Mar 1, 1978·The Journal of Experimental Medicine·R M ZinkernagelJ Klein
Sep 1, 1978·The Journal of Experimental Medicine·P J Fink, M J Bevan
Feb 8, 1979·Nature·B J MathiesonR Asofsky
Jan 1, 1978·Advances in Cancer Research·R B Herberman, H T Holden
Jun 1, 1975·The Journal of Experimental Medicine·H Cantor, E A Boyse
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Mar 10, 1971·Nature: New Biology·C P StannersH Green
Jul 1, 1980·The Journal of Experimental Medicine·S Muraoka, R G Miller
Apr 1, 1980·The Journal of Experimental Medicine·H Ishikawa, K Saito
Citations
Nov 1, 1983·The Journal of Experimental Medicine·J Reimann, R G Miller
Jan 1, 1991·Microbiology and Immunology·M J Tanabe
Mar 1, 1985·Cell·J Reimann, R G Miller
Jun 1, 1986·Immunological Reviews·O Stutman
Jan 1, 1984·International Journal of Cell Cloning·P Goube de Laforest, L A Rozenszajn
Jan 1, 1984·International Journal of Cell Cloning·M D MossalayiJ Tanzer
Oct 1, 1983·Immunology Today·R Scollay