PMID: 61042Sep 13, 1976Paper

Generation of electric current by chromatophores of Rhodospirillum rubrum and reconstitution of electrogenic function in subchromatophore pigment-protein complexes

Biochimica Et Biophysica Acta
L A DrachevV P Skulachev

Abstract

Lipoprotein complexes, containing (1) bacteriochlorophyll reaction centers, (2) bacteriochlorophyll light-harvesting antenna or (3) both reaction centers and antenna, have been isolated from chromatophores of non-sulphur purple bacteria Rhodospirillum rubrum by detergent treatments. The method of reconstituting the proteoliposomes containing these complexes is described. Being associtated with planas azolectin membrane, ptoteoliposomes as well as intact chromatophores were found to generate a light-dependent transmembrane electric potential difference measured by Ag/AgC1 electrodes and voltmeter. The direction of the electric field inproteoliposomes can be regulated by the addition of antenna complexes to the reconstitution mixture. The reaction center complex proteoliposomes generate an electric field of a direction opposite to that in chromatophores, whereas proteoliposomes containing reaction center complexes and a sufficient amount of antenna complexes produce a potential difference as in chromatophores. ATP and inorganic pyrophosphate, besides light, were shown to be usable as energy sources for electric generation in chromatophores associated with planar membrane.

References

Aug 4, 1970·Biochimica Et Biophysica Acta·L L GriniusM A Vladimirova
Jan 1, 1972·Biochimica Et Biophysica Acta·L A Sherman, W S Cohen
Oct 17, 1968·European Journal of Biochemistry·J B JacksonL V von Stedingk
Apr 1, 1969·European Journal of Biochemistry·P ScholesJ Moyle
Aug 1, 1969·FEBS Letters·J B. Jackson, A R. Crofts
Feb 1, 1957·Journal of Cellular Physiology·G COHEN-BAZIRER Y STANIER
Jul 1, 1963·Journal of Medicinal Chemistry·J P DICKIEF M STRONG

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Citations

Jun 1, 1977·Journal of Bioenergetics and Biomembranes·N P Krasinskaya, V D Samuilov
Feb 1, 1981·Quarterly Reviews of Biophysics·M MontalH Schindler
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M SchönfeldG Feher
Sep 14, 1977·The Journal of Membrane Biology·S B HwangW Stoeckenius
Sep 14, 1977·The Journal of Membrane Biology·S B HwangW Stoeckenius
Sep 26, 2013·Biochimica Et Biophysica Acta·Christian BamannClemens Glaubitz
Oct 15, 1977·FEBS Letters·V I Godik, A Y Borisov
Feb 1, 1993·Quarterly Reviews of Biophysics·E BambergK Fendler
Jul 1, 1981·European Journal of Biochemistry·L A Drachev Uspenskaya NYa
Dec 1, 1980·European Journal of Biochemistry·A A KondrashinV P Skulachev
Nov 16, 2020·Journal of Bioenergetics and Biomembranes·Andrey A ZaspaMahir D Mamedov
Nov 5, 1980·Biochimica Et Biophysica Acta·R PicorelG Gingras
Jul 1, 1979·Analytical Biochemistry·L A DrachevV P Skulachev

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