Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines

Current Protocols in Immunology
G Tosato, Jeffrey I Cohen

Abstract

Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.

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Citations

Mar 5, 2013·Immunogenetics·Jean-François GélinasNaglaa H Shoukry
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May 6, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhao LiPengfei Wang
May 21, 2016·Science·Erlend StrønenTon N Schumacher
Jan 24, 2021·Proceedings of the National Academy of Sciences of the United States of America·Wentao ChenJames A Wells

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