PMID: 9170387May 1, 1997Paper

Generation of free radicals during anoxia and reoxygenation in perfused osteoblastlike cells

Clinical Orthopaedics and Related Research
A GasbarriniA Facchini

Abstract

Sensitivity to ischemia and reperfusion injury is a main problem afflicting tissues exposed to a prolonged period of oxygen deprivation. The generation of oxygen free radicals, in particular, is considered a major cause of postischemic reperfusion injury. However, studies on the mechanisms of production of free radicals are limited by the difficulty to measure in real time their formation and to discriminate between the different oxyradical species. The aim of this study was to determine whether the formation of oxygen free radicals occurs in murine osteoblastlike cells (MC3T3-E1) exposed to anoxia and reoxygenation and to explore its relation to the reoxygenation injury. Cells were cast in agarose and perfused with oxygenated Krebs-Henseleit bicarbonate buffer. Anoxia was obtained by shifting the gas phase of the media to 95% N2-5% CO2. Oxygen free radicals were detected by enhanced chemiluminescence: anion superoxide or hydrogen peroxide was measured by adding lucigenin or luminol plus horseradish peroxidase to the media, respectively. Cell injury was assessed by the rate of lactate dehydrogenase release. During the control period, lucigenin and luminol plus horseradish chemiluminescences were 15 +/- 1 nA per chamber and 20 +...Continue Reading

References

Nov 1, 1989·Journal of Applied Physiology·S L ArcherE K Weir
Jan 1, 1988·Free Radical Biology & Medicine·J T Flaherty, M L Weisfeldt
Oct 1, 1984·Experimental Cell Research·D L FoxallJ B Mitchell
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A BoverisB Chance

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Citations

May 11, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Yan-Liang ZhangMirianas Chachisvilis
Sep 8, 2017·Journal of Dental Anesthesia and Pain Medicine·Ji-Young YoonJeong-Hoon Park

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