Generation of fully pluripotent female murine-induced pluripotent stem cells

Biology of Reproduction
Ke-Qian DiJian-Hui Tian

Abstract

The high quality of induced pluripotent stem cells (iPSCs) has been determined to be high-grade chimeras that are competent for germline transmission, and viable mice can be generated through tetraploid complementation. Most of the high-quality iPSCs described to date have been male. Female iPSCs, especially fully pluripotent female iPSCs, are also essential for clinical applications and scientific research. Here, we show, for the first time, that a gender-mixed induction strategy could lead to a skewed sex ratio of iPSCs. After reprogramming, 50%, 70%, and 90% female initiating mouse embryonic fibroblasts at different male ratios resulted in 14.1 ± 6.8% (P < 0.05), 31.8 ± 5.4% (P < 0.05), and 80.1 ± 2.8% (P < 0.05) female iPSCs, respectively. Furthermore, these female iPSCs had pluripotent properties typical of embryonic stem cells. Importantly, these fully pluripotent female iPSCs could generate viable mice by tetraploid complementation. These findings indicate that high-quality female iPSCs could be derived effectively, and suggest that clinical application of female iPSCs is feasible.

References

Jan 10, 1997·Experimental Cell Research·A K VossP Gruss
Dec 13, 2005·Nature Genetics·Di Kim Nguyen, Christine M Disteche
Jan 19, 2007·Nature Clinical Practice. Cardiovascular Medicine·Georgina M EllisonBernardo Nadal-Ginard
Jun 8, 2007·Nature·Keisuke OkitaShinya Yamanaka
Mar 29, 2008·Cell Stem Cell·Matthias StadtfeldKonrad Hochedlinger
May 30, 2008·Nature·Tarjei S MikkelsenAlexander Meissner
Aug 30, 2008·Annual Review of Genetics·Bernhard Payer, Jeannie T Lee
Sep 20, 2008·Science·Pablo NavarroPhilip Avner
Oct 14, 2008·Nature Biotechnology·Danwei HuangfuDouglas A Melton
Feb 5, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carlos PenalozaZahra Zakeri
Jun 25, 2009·Stem Cell Reviews and Reports·Wouter Scheper, Sjef Copray
Aug 13, 2009·Nature·Michael J BolandKristin K Baldwin
Dec 29, 2009·Cell Stem Cell·Miguel Angel EstebanDuanqing Pei
Apr 30, 2010·Biology of Reproduction·Zhaohui KouShaorong Gao
Feb 16, 2011·Cell Research·Bernadett Papp, Kathrin Plath
Mar 8, 2011·Seminars in Cell & Developmental Biology·Daniel H KimJeannie T Lee
Mar 19, 2011·Nature Reviews. Genetics·Kathrin Plath, William E Lowry
Oct 12, 2012·Cellular and Molecular Life Sciences : CMLS·Tatsuya Ohhata, Anton Wutz
May 15, 2013·Annual Review of Genomics and Human Genetics·Derek LessingJeannie T Lee
Aug 2, 2013·Nature·Elizabeth Pollitzer

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Citations

Feb 20, 2016·Transgenic Research·Delia A Soto, Pablo J Ross
Apr 17, 2020·Biology of Sex Differences·Joshua B RubinJingqin Luo

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Methods Mentioned

BETA
PCR
fluorescence microscopy
Cesarean section

Software Mentioned

Quantity One
Statistical Package for the Social Sciences ( SPSS ) SPSS

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