Generation of functional dendritic cells for potential use in the treatment of acute lymphoblastic leukemia

Cancer Immunology, Immunotherapy : CII
Dagmar PospísilováJirina Bartůnková

Abstract

Immunotherapy of malignant diseases mediated by dendritic cells (DC) pulsed with tumor antigens ex vivo is a promising new tool in the individual treatment of malignant diseases. The present study focuses on the problem of how to optimize in vitro culture conditions and induce the maturation of DC with the capacity to induce antitumor immunity toward leukemic cells. DC were generated from peripheral mononuclear cells by co-cultivation with granulocyte/macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). Tumor antigens were added for 2 h after 7 days in culture. Irradiated leukemic blasts, blast lysate, apoptotic cells from the Jurkat cell line (T ALL) and their lysate were used in various concentrations for antigen pulsing. Harvested DC were phenotyped by flow cytometry, and viability was assessed using trypan blue exclusion (Annexin test). After the cells had been pulsed with tumor antigens and co-cultured with autologous lymphocytes, the production of interferon-gamma (IFN-gamma) and IL-12 was analyzed, and lymphocyte proliferative response and cytotoxicity against the target tumor cell line were assessed. The cultivation of monocytes under the described conditions led to the expression of surface markers t...Continue Reading

Citations

Jan 14, 2003·Hematological Oncology
May 12, 2004·Immunology Letters·Taku FujimuraTakashi Nishimura
Jan 3, 2012·Journal of Translational Medicine·Jitka FučíkováRadek Špíšek
Dec 25, 2003·Leukemia & Lymphoma·E R KaminskiA G Prentice
Apr 24, 2009·The Journal of Investigative Dermatology·Mark J BluthJohn A Carucci
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