PMID: 6976408Dec 1, 1981Paper

Generation of functional human T cell hybrids

The Journal of Experimental Medicine
O IrigoyenL Chess

Abstract

Human T cell hybrids were generated by fusing lectin-activated normal and leukemic human T cells with an aminopterin-sensitive human T cell line. This mutant cell line, designated CEM-T15, was derived from the human T cell line CEM after chemical mutagenesis with ethane methylsulfonate and subsequent culture in medium containing 6-thioguanine. After polyethylene glycol-induced fusion, the cells were cultured in hypoxanthine-aminopterin-thymidine selective medium. More than 5 wk after fusion, evidence for successful hybridization was obtained by three independent criteria: (a) The majority of the cultures contained cells expressing the OKT3 surface antigen: this antigen is expressed on normal T cells but not on CEM-T15 cells. (b) Most of the cultures contained polyploid cells. (c) Some of the cultures provided helper activity in the generation of antibody-forming cells. This functional activity is absent from the CEM-T15 parental cell line. Evidence for functional stability of the hybrids greater than 20 wk after fusion was provided by several clones that not only continue growing exponentially but also maintain expression of OKT3 surface antigen and high levels of helper function. These T cell hybrids constructed using antigen-...Continue Reading

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Citations

Sep 1, 1984·Journal of Clinical Immunology·A MuraguchiA S Fauci
Jan 1, 1994·International Journal of Clinical & Laboratory Research·M T ScupoliR S Accolla
Oct 26, 1984·Journal of Immunological Methods·K SugamuraY Hinuma
Feb 28, 1985·Journal of Immunological Methods·N KouttabA L Maizel
Aug 2, 1985·Journal of Immunological Methods·L MayerH G Kunkel
Mar 1, 1990·Veterinary Immunology and Immunopathology·J O Olobo, S J Black
Apr 3, 1982·Lancet
Apr 3, 1982·Lancet
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E C DeFreitasC M Croce
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S K FoungE G Engleman
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J LeW Prensky
Jan 1, 1983·The Journal of Experimental Medicine·J L ButlerA S Fauci
Jun 30, 1983·Annals of the New York Academy of Sciences·L MayerH G Kunkel
Jun 15, 1983·International Journal of Cancer. Journal International Du Cancer·D ZaguryM Feldman
Apr 15, 1983·International Journal of Cancer. Journal International Du Cancer·D ZaguryM Feldman

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