Generation of functional human vascular network

Transplantation Proceedings
Takanori TakebeHideki Taniguchi

Abstract

One of the major obstacles in regenerating thick, complex tissues such as the liver is their need for vascularization, which is essential to maintain cell viability during tissue growth and to induce structural organization. Herein, we have described a method to engineer a functional human vascular network. Enhanced green fluorescence protein-labeled human umbilical vein endothelial cells (GFP-HUVECs) were cocultivated with kusabira orange-labeled human mesenchymal stem cells (KO-hMSCs) inside a collagen/fibronectin matrix. Premature vascular network formation was visualized by fluorescence microscopy imaging. Furthermore, constructs prevascularized in vitro were implanted into a transparency window in immunodeficient mice. Following several days of cultivation, GFP-HUVECs formed vessel-like structures that were stabilized by pericytes differentiated from KO-hMSCs. After implantation in vivo, the patency of human vascular structures was proved by rhodamine dextran infusion. These functional vascular structures remained for over 2 months. Vascularization is the key challenge to organ generation. We successfully generated human vascular networks inside a matrix. Integration of parenchymal cells using our engineering technique sho...Continue Reading

Citations

Sep 10, 2014·The Journal of Clinical Investigation·Takanori TakebeHideki Taniguchi
Jul 18, 2014·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Aleem Ahmed KhanJ Venkateshwarulu
Jan 1, 2013·Cell Regeneration·Yunying LiuWei-Qiang Gao
May 7, 2013·Annual Review of Biomedical Engineering·François A AugerDan Lacroix
Oct 17, 2014·Thrombosis and Haemostasis·C PatraF B Engel
Jun 6, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Rebecca JeyarajAaron Tan
Jan 24, 2014·Organogenesis·Takanori TakebeHideki Taniguchi
Sep 25, 2018·Regenerative Medicine·Yunqing Kang, Jia Chang
Jun 23, 2016·Current Stem Cell Reports·Oriane B MatthysTodd C McDevitt

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