Nov 28, 2017

Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System

Methods in Molecular Biology
Paul J Baker, Seth L Masters

Abstract

CRISPR/Cas9-based gene targeting allows deletion of a gene of interest from cultured cell lines. Due to difficulty in transiently transfecting hematopoetic cells with components required for this process, we have adopted a lentiviral system for delivery of the CRISPR/Cas9 components into myeloid cell lines. Here, we detail the process of knocking out genes from pools of cultured myeloid cells using this CRISPR/Cas9 system and describe methods of validating these knockout pools.

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Mentioned in this Paper

Subfamily lentivirinae
Shuttle Vectors
Transfection
Genes
CRISPR-Cas Systems
Knock-out
Gene Deletion Abnormality
Gene Deletion
Myeloid Cells
Myeloid Progenitor Cells

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