Generation of glycerophospholipid molecular species in the yeast Saccharomyces cerevisiae. Fatty acid pattern of phospholipid classes and selective acyl turnover at sn-1 and sn-2 positions

Yeast
S Wagner, Fritz Paltauf

Abstract

Acyl chains linked to phospholipids of the yeast, Saccharomyces cerevisiae, are mainly C16:1 and C18:1 accompanied by minor amounts of C14:0, C16:0 and C18:0. In view of this rather simple fatty acid composition, the question arose whether in yeast, as in higher eukaryotes, fatty acyl groups were characteristically distributed among the sn-1 and sn-2 positions of distinct phospholipid classes. Analysis of fatty acids linked to the sn-1 and sn-2 positions of the major phospholipids showed that indeed saturated fatty acyl groups predominated in the sn-1 positions. While the percentage of saturated fatty acids was low (10%) in phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) from cells grown on rich medium, it was higher in phosphatidylserine (PtdSer) (25%) and highest in phosphatidylinositol (PtdIns) (41%). Oleate was mainly linked to position sn-2, while palmitoleate predominated in position sn-1. Striking differences in the fatty acid distribution of phospholipids that are metabolically closely related (e.g. PtdSer and PtdEtn, PtdEtn and PtdCho, and PtdIns and PtdSer) suggest that pathways must exist for the generation of distinct phospholipid molecular species within the different phospholipid classes. The hi...Continue Reading

References

Jul 9, 1991·Biochimica Et Biophysica Acta·J I MacDonald, H Sprecher
Sep 1, 1966·Journal of Bacteriology·M A Resnick, R K Mortimer

Citations

Dec 12, 2002·Molecular Biology of the Cell·Verena TatzerR Schneiter
Feb 11, 2012·Molecular Biology of the Cell·Cedric H De SmetAnton I P M De Kroon
Jul 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ingo HeilmannJohn Shanklin
Sep 25, 2004·Molecular Microbiology·Hiromi Sato, Dara W Frank
Aug 22, 2006·Biochimica Et Biophysica Acta·Maria L GasparSusan A Henry
Aug 15, 2014·Biotechnology and Bioengineering·Xiaoling Tang, Wei Ning Chen
Mar 18, 2000·Methods : a Companion to Methods in Enzymology·K Matsuoka, R Schekman
Apr 29, 2015·Biochimica Et Biophysica Acta·Anna WerleinGerhard Pütz
May 20, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Panneerselvam VijayarajVasanthi Nachiappan
Jan 11, 2014·Biochimica Et Biophysica Acta·Mariam AyyashPeter Oelkers
May 2, 2013·Progress in Lipid Research·Anton I P M De KroonCedric H De Smet
May 14, 2003·Applied Microbiology and Biotechnology·D Sorger, G Daum
Jan 29, 2016·Lipid Insights·Mike F RenneAnton I P M De Kroon
Aug 7, 2007·The Journal of Biological Chemistry·Mohammed BenghezalAndreas Conzelmann
Oct 11, 2019·Scientific Reports·Antonisamy William JamesVasanthi Nachiappan
Jul 1, 2020·Essays in Biochemistry·Sang-Chul Kim, Xuemin Wang
Sep 20, 2020·International Journal of Molecular Sciences·Fumio SakaneChiaki Murakami

Related Concepts

Acylation
Cell Extracts
Saturated Fat
Lysophospholipids
Oleic Acids
Palmitic Acids
Diacylglycerophosphates
Phospholipases A
Saccharomyces cerevisiae
Phospholipases A2

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