Nov 2, 2014

Generation of haploid spermatids with fertilization and development capacity from human spermatogonial stem cells of cryptorchid patients

Stem Cell Reports
Shi YangZuping He

Abstract

Generation of functional spermatids from azoospermia patients is of unusual significance in the treatment of male infertility. Here, we report an efficient approach to obtain human functional spermatids from cryptorchid patients. Spermatogonia remained whereas meiotic germ cells were rare in cryptorchid patients. Expression of numerous markers for meiotic and postmeiotic male germ cells was enhanced in human spermatogonial stem cells (SSCs) of cryptorchidism patients by retinoic acid (RA) and stem cell factor (SCF) treatment. Meiotic spreads and DNA content assays revealed that RA and SCF induced a remarkable increase of SCP3-, MLH1-, and CREST-positive cells and haploid cells. Single-cell RNA sequencing analysis reflected distinct global gene profiles in embryos derived from round spermatids and nuclei of somatic cells. Significantly, haploid spermatids generated from human SSCs of cryptorchid patients possessed fertilization and development capacity. This study thus provides an invaluable source of autologous male gametes for treating male infertility in azoospermia patients.

  • References54
  • Citations31

Mentioned in this Paper

Embryo
Pachytene
Real-Time Polymerase Chain Reaction
Human Chorionic Gonadotropin
Flow Cytometry
Biochemical Pathway
Benign Neoplasm of Testis
GFRA1 gene
Y Chromosome
TNP1 gene

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