PMID: 11339423May 8, 2001Paper

Generation of hepatitis C virus-specific cytotoxic T lymphocytes from healthy individuals with peptide-pulsed dendritic cells

Journal of Gastroenterology and Hepatology
A ItoN Hayashi

Abstract

In hepatitis C virus (HCV) infection, cytotoxic T lymphocytes (CTL) are involved in liver inflammation and contribute to the reduction of viral load. Antibodies for HCV-CTL precursor frequencies (CTLpf) are relatively low in chronic hepatitis C, and this may be related to the poor CTL response in vivo. The aim of this study was to assess the efficacy of dendritic cells (DC) as antigen-presenting cells in CTL generation from low CTLpf. To confirm the rationale of using DC to prime naive T cells, five HCV-uninfected individuals were enrolled in the study. We obtained DC by maturation from peripheral progenitors under stimulation with granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-4 and IL-1alpha. Autologous T cells were cultured with DC or concanavalin-A-induced blasts loaded with four HCV-derived peptides bearing human leukocyte antigen (HLA)-A*0201 or -A24 motifs for 28 days under IL-7 and IL-2 stimulation. The lytic activity against peptide-pulsed targets was assessed by using a [51Cr]-releasing assay. The DC strongly expressed HLA class I, II, B7-1 and B7-2, but not phenotypic markers of T-, B-, natural killer (NK)-cells or monocytes. The CD8-positive, HLA-class I-restricted and HCV peptide-specif...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·R M Steinman
Oct 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K KiyosawaR H Purcell
Nov 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H KitaM Imawari
Feb 1, 1995·European Journal of Immunology·A CernyF V Chisari
Jan 1, 1995·Immunogenetics·H G RammenseeS Stevanoviíc
Jul 1, 1994·The Journal of Experimental Medicine·N RomaniG Schuler
Aug 1, 1996·The Journal of Experimental Medicine·D BoczkowskiE Gilboa
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K HiroishiM Imawari
Jan 4, 1998·Journal of Gastroenterology and Hepatology·M TomimatsuH Hisatomi

❮ Previous
Next ❯

Citations

May 3, 2003·Methods : a Companion to Methods in Enzymology·David M Koelle
Jun 12, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ola WeilandMatti Sällberg
Jan 22, 2003·Immunology and Cell Biology·Nina L FowlerEric J Gowans
May 3, 2002·Clinical and Experimental Immunology·S WardP Klenerman
Jan 6, 2009·Uirusu·Takashi EbiharaTsukasa Seya
Oct 29, 2013·Pathology Oncology Research : POR·János HunyadiEva Rajnavölgyi
Feb 18, 2011·Journal of Gastroenterology·Angela Dolganiuc, Gyongyi Szabo
Feb 5, 2005·Cancer Immunology, Immunotherapy : CII·Katharina Gabriele HüttnerThomas Felzmann
Apr 25, 2006·Journal of Biomedical Science·Lih-Hwa Hwang
Apr 29, 2009·Journal of Cancer Research and Clinical Oncology·Long WuYan Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.