Generation of hermaphrodite transgenic papaya lines with virus resistance via transformation of somatic embryos derived from adventitious roots of in vitro shoots.

Transgenic Research
Yi-Jung KungShyi-Dong Yeh

Abstract

Papaya production is seriously limited by Papaya ringspot virus (PRSV) worldwide and Papaya leaf-distortion mosaic virus (PLDMV) in Eastern Asia. An efficient transformation method for developing papaya lines with transgenic resistance to these viruses and commercially desirable traits, such as hermaphroditism, is crucial to shorten the breeding program for this fruit crop. In this investigation, an untranslatable chimeric construct pYP08 containing truncated PRSV coat protein (CP) and PLDMV CP genes coupled with the 3' untranslational region of PLDMV, was generated. Root segments from different portions of adventitious roots of in vitro multiple shoots of hermaphroditic plants of papaya cultivars 'Tainung No. 2', 'Sunrise', and 'Thailand' were cultured on induction medium for regeneration into somatic embryos. The highest frequency of somatic embryogenesis was from the root-tip segments of adventitious roots developed 2-4 weeks after rooting in perlite medium. After proliferation, embryogenic tissues derived from somatic embryos were wounded in liquid-phase by carborundum and transformed by Agrobacterium carrying pYP08. Similarly, another construct pBG-PLDMVstop containing untranslatable CP gene of PLDMV was also transferred t...Continue Reading

References

Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N. UrasakiR. Terauchi
Apr 1, 1994·Plant Cell Reports·M MondalB B Mukherjee
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May 1, 2004·Plant Disease·Gustavo FerminDennis Gonsalves

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Citations

Mar 3, 2012·Transgenic Research·Giorgio Gambino, Ivana Gribaudo
Mar 21, 2021·Journal of Plant Physiology·Xiaobing ZhaoJingjing Yue

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