Generation of human induced pluripotent stem cells from a Bombay individual: moving towards "universal-donor" red blood cells

Biochemical and Biophysical Research Communications
Ali SeifinejadHossein Baharvand

Abstract

Bombay phenotype is one of the rare phenotypes in the ABO blood group system that fails to express ABH antigens on red blood cells. Nonsense or missense mutations in fucosyltransfrase1 (FUT1) and fucosyltransfrase2 (FUT2) genes are known to create this phenotype. This blood group is compatible with all other blood groups as a donor, as it does not express the H antigen on the red blood cells. In this study, we describe the establishment of human induced pluripotent stem cells (iPSCs) from the dermal fibroblasts of a Bombay blood-type individual by the ectopic expression of established transcription factors Klf4, Oct4, Sox2, and c-Myc. Sequence analyses of fibroblasts and iPSCs revealed a nonsense mutation 826C to T (276 Gln to Ter) in the FUT1 gene and a missense mutation 739G to A (247 Gly to Ser) in the FUT2 gene in the Bombay phenotype under study. The established iPSCs resemble human embryonic stem cells in morphology, passaging, surface and pluripotency markers, normal karyotype, gene expression, DNA methylation of critical pluripotency genes, and in-vitro differentiation. The directed differentiation of the iPSCs into hematopoietic lineage cells displayed increased expression of the hematopoietic lineage markers such as C...Continue Reading

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Citations

Jan 5, 2011·Antioxidants & Redox Signaling·Ji Woong Han, Young-Sup Yoon
Aug 4, 2011·International Journal of Cell Biology·Laura J NortonMerlin Crossley
Jan 18, 2011·European Journal of Internal Medicine·Giuseppe LippiMassimo Franchini
Jan 8, 2011·British Journal of Haematology·Claude BagnisPascal Bailly
Feb 16, 2010·British Journal of Haematology·Jo MountfordMarc Turner
May 9, 2012·Stem Cells International·Carolyn WhitsettGiuliano Grazzini
Apr 28, 2018·EMBO Molecular Medicine·Joseph HawksworthAshley M Toye
Oct 30, 2018·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Min SongHua Lu

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