PMID: 3760859Jul 1, 1986Paper

Generation of hydrogen peroxide by incidental metal ion-catalyzed autooxidation of glutathione

Journal of Inorganic Biochemistry
P W AlbroJ L Schroeder

Abstract

Autooxidation of reduced glutathione in 50 mM buffer at pH 7.9 is indetectably slow in the presence of 1 mM DETAPAC, EDTA, TET, or tripyridine, but passing buffer through Chelex resin was insufficient to remove traces of catalytically active metals. Production of hydrogen peroxide during glutathione autooxidation was catalyzed by traces of Fe+2 or Cu+2, and to a much lesser extent by Cu+1 and Ni+2, but not to a detectable extent by Na+1, K+1, Fe+3, Al+3, Cd+2, Zn+2, Ca+2, Mg+2, Mn+2, or Hg+2. Cysteine was a much better precursor for hydrogen peroxide production than were cysteine sulfinic or sulfonic acids. The chelators EGTA, NTA, bipyridine, dimethyl glyoxime, salicylate, and Desferal were ineffective at preventing autooxidation. EDDA and 8-hydroxyquinoline were partially effective. Catalase could completely prevent the accumulation of detectable H2O2, but superoxide dismutase was only slightly inhibitory. Hydroxyl radical and singlet oxygen quenching agents (mannitol and histidine) stimulated. A mechanism for the production of H2O2 during trace metal catalyzed oxidation of glutathione is proposed, involving glutathione-complexed metal and dissolved oxygen. Although a radical intermediate can not be ruled out, no radical init...Continue Reading

Citations

Oct 1, 1987·Lipids·P W AlbroJ L Schroeder
Jun 1, 1989·Journal of Biochemical and Biophysical Methods·J T Corbett
Jan 1, 1990·Free Radical Biology & Medicine·D M MillerS D Aust
May 3, 2012·Journal of the American Chemical Society·Christopher F MonsonPaul S Cremer
Nov 6, 2002·The Plant Cell·Surasak SiripornadulsilRichard T Sayre
Mar 1, 1996·European Journal of Biochemistry·A CorazzaP J Sadler
Feb 6, 2007·Journal of Inorganic Biochemistry·Anshul Gupte, Russell J Mumper
Jul 12, 2005·Bioorganic & Medicinal Chemistry Letters·Tonika ChatterjiKent S Gates
Nov 21, 2002·Analytical Biochemistry·Jolanta GłebskaGrzegorz Bartosz
Aug 4, 1998·Free Radical Research·A V KachurJ E Biaglow
Dec 13, 2017·Chemical Communications : Chem Comm·Maryam Imani NejadKent S Gates
Apr 29, 1998·Journal of Chromatography. a·A DegenerW H Velander

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