PMID: 2494288Mar 1, 1989Paper

Generation of hydrogen peroxide in cultured porcine thyroid cells: synergistic regulation by cytoplasmic free calcium and protein kinase C

The Journal of Endocrinology
N TakasuI Komiya

Abstract

This study set out to elucidate the mechanism by which H2O2 generation is regulated in cultured porcine thyroid cells. We monitored continuously the effects of the calcium ionophore A23187 and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on H2O2 generation, using homovanillic acid and horseradish peroxidase. A23187 and TPA stimulated H2O2 generation. A23187 increased cytoplasmic free calcium and TPA activated protein kinase C. Generation of H2O2 is therefore regulated by cytoplasmic free calcium and protein kinase C. Exposure to A23187 or TPA augmented further the stimulation of H2O2 generation by TPA or A23187 respectively. Thus A23187 and TPA, by increasing cytoplasmic free calcium and activating protein kinase C respectively, synergistically activate H2O2 generation.

Citations

Apr 28, 1995·Molecular and Cellular Endocrinology·U Björkman, R Ekholm
Jan 16, 2003·Cell Calcium·Maria Giovanna EliaSanto Marsigliante
Aug 19, 2007·The Journal of Clinical Investigation·Jukka KeroStefan Offermanns
Sep 29, 2011·Cellular and Molecular Life Sciences : CMLS·Karen A M KennedyShawn S-C Li
Jan 1, 1992·Progress in Histochemistry and Cytochemistry·E Raspé, J E Dumont
Jan 1, 2008·Free Radical Biology & Medicine·Albert van der Vliet
Sep 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K L SungW H Akeson
Feb 2, 2002·Experimental Cell Research·Xavier De DekenFrançoise Miot

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