PMID: 7334020Dec 1, 1981Paper

Generation of hydrogen peroxide on oxidation of NADH by hepatic plasma membranes

Journal of Bioenergetics and Biomembranes
T RamasarmaF L Crane

Abstract

The oxidation of NADH by mouse liver plasma membranes was shown to be accompanied by the formation of H2O2. The rate of H2O2 formation was less than one-tenth the rate of oxygen uptake and much slower than the rate of reduction of artificial electron acceptors. The optimum pH for this reaction was 7.0 and the Km value for NADH was found to be 3 X 10(-6) M. The H2O2-generating system of plasma membranes was inhibited by quinacrine and azide, thus distinguishing it from similar activities in endoplasmic reticulum and mitochondria. Both NADH and NADPH served as substrates for plasma membrane H2O2 generation. Superoxide dismutase and adriamycin inhibited the reaction. Vanadate, known to stimulate the oxidation of NADH by plasma membranes, did not increase the formation of H2O2. In view of the growing evidence that H2O2 can be involved in metabolic control, the formation of H2O2 by a plasma membrane NAD(P)H oxidase system may be pertinent to control sites at the plasma membrane.

References

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Oct 1, 1976·FEBS Letters·F L Crane, H Löw
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Citations

Jun 1, 1991·Journal of Bioenergetics and Biomembranes·D J Morré, A O Brightman
Oct 1, 1991·Journal of Bioenergetics and Biomembranes·F L CraneH Löw
Jan 1, 1994·Molecular Aspects of Medicine·F L CraneH Löw
Aug 1, 1985·Biochimica Et Biophysica Acta·F L CraneH Löw
Nov 11, 2008·Biochemistry. Biokhimii︠a︡·V D SamuilovL A Vasil'ev
Mar 9, 2012·Biochemistry Research International·Frederick L Crane, Hans Löw
Jan 24, 2007·Physiological Reviews·Karen Bedard, Karl-Heinz Krause
Jan 5, 2002·Journal of the American College of Nutrition·F L Crane
Jan 28, 2021·International Journal of Molecular Sciences·Rayan Bou-FakhredinAli T Taher
Aug 11, 1982·Biochimica Et Biophysica Acta·T Ramasarma

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