Generation of influenza virus from avian cells infected by Salmonella carrying the viral genome

PloS One
Xiangmin ZhangRoy Curtiss

Abstract

Domestic poultry serve as intermediates for transmission of influenza A virus from the wild aquatic bird reservoir to humans, resulting in influenza outbreaks in poultry and potential epidemics/pandemics among human beings. To combat emerging avian influenza virus, an inexpensive, heat-stable, and orally administered influenza vaccine would be useful to vaccinate large commercial poultry flocks and even migratory birds. Our hypothesized vaccine is a recombinant attenuated bacterial strain able to mediate production of attenuated influenza virus in vivo to induce protective immunity against influenza. Here we report the feasibility and technical limitations toward such an ideal vaccine based on our exploratory study. Five 8-unit plasmids carrying a chloramphenicol resistance gene or free of an antibiotic resistance marker were constructed. Influenza virus was successfully generated in avian cells transfected by each of the plasmids. The Salmonella carrier was engineered to allow stable maintenance and conditional release of the 8-unit plasmid into the avian cells for recovery of influenza virus. Influenza A virus up to 10⁷ 50% tissue culture infective doses (TCID50)/ml were recovered from 11 out of 26 co-cultures of chicken embr...Continue Reading

References

Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Jan 1, 1991·Archives of Virology·T M ChambersR G Webster
Jul 1, 1991·Virology·U SchultzC Scholtissek
Dec 1, 1990·The Journal of Infectious Diseases·H A Lockman, R Curtiss
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·W Schaffner
Jul 1, 1983·Applied and Environmental Microbiology·S G RogersM J Brackin
Nov 1, 1981·Infection and Immunity·V S HinshawW J Bean
Feb 1, 1997·Experimental Cell Research·D A Dean
Oct 23, 1997·Nature·J C de JongW L Lim
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J HessS H Kaufmann
Sep 22, 1998·Nature Biotechnology·C Grillot-CourvalinP Courvalin
Nov 21, 1998·Journal of Biotechnology·M DrögeW Selbitschka
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A ZantaJ P Behr
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G NeumannY Kawaoka
Sep 3, 1999·Nature Biotechnology·A SubramanianS L Diamond
Dec 10, 1999·Experimental Cell Research·D A DeanL C Smith
May 9, 2000·Veterinary Microbiology·T Ito, Y Kawaoka
May 12, 2000·Science·A D OsterhausR A Fouchier
May 18, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·A MesikaZ Reich
Jan 10, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R J Webby, R G Webster
Mar 22, 2002·Cellular Microbiology·Catherine Grillot-CourvalinPatrice Courvalin
Jul 15, 2003·Journal of Virology·Luka Cicin-SainUlrich H Koszinowski

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Citations

Mar 10, 2016·Current Opinion in Biotechnology·Marie BeitelsheesBlaine A Pfeifer
Dec 20, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Josephine E Clark-Curtiss, Roy Curtiss

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Methods Mentioned

BETA
PCR
transfection
electrophoresis
genetic modifications

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