PMID: 11334969May 4, 2001Paper

Generation of large numbers of human dendritic cells from whole blood passaged through leukocyte removal filters: an alternative to standard buffy coats

Journal of Immunological Methods
S EbnerC Heufler

Abstract

Many blood banks now use whole blood inline filtration to produce leukocyte-depleted blood products. As a result, a common source of large numbers of human dendritic cells (DC) for research purposes, namely standard buffy coats, has been lost. Therefore, we have adapted our conventional method for growing DC from CD14(+) precursors in order to make use of these filter units. A dextran solution containing human serum albumin was used to flush back the filters. After pelleting, mononuclear cells were obtained by standard density gradient centrifugation (Lymphoprep). To eliminate T cells, we used rosetting with sheep red blood cells. In addition to the classical PBMC, the cell population obtained after Lymphoprep centrifugation was found to contain high numbers of CD14(+) granulocytes which could be depleted by separation on an additional Percoll gradient. At this stage, FACS analysis revealed a cell population that resembled the CD14(+) monocyte-enriched population, obtained from traditional buffy coat preparations after Lymphoprep centrifugation and T cell elimination. Culture of the cells and the induction of maturation was identical to the previously described procedures, except that the culture time was reduced from 7 to 5 da...Continue Reading

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Aug 23, 2002·Journal of Immunological Methods·Sandra TuyaertsKris Thielemans
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Feb 10, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Martin TiefenthalerChristine Heufler
Dec 3, 2014·Journal of Immunological Methods·Mikaela M Tremblay, Jon C D Houtman
Jul 26, 2003·The Journal of Investigative Dermatology·Susanne HoferChristine Heufler
Jul 29, 2015·Journal of Cellular and Molecular Medicine·Beate PoschChristine Heufler
Jan 25, 2014·Journal of Immunological Methods·Anna KoryakinaPeter Bruegger
Sep 21, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniela OrtnerChristine Heufler
Jan 11, 2021·Hematology, Transfusion and Cell Therapy·Shirin FerdowsiAli Akbar Pourfathollah
Jul 23, 2019·The Journal of Allergy and Clinical Immunology·Dominik KlaverChristine Heufler
Apr 29, 2020·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·N SasaniA Aghaie

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