PMID: 2113464May 1, 1990Paper

Generation of lipoxygenase metabolites of arachidonic acid by monolayer cultures of tracheal epithelial cells and intact tracheal segments from rabbits

Experimental Lung Research
S E AlpertM K Bach

Abstract

We compared the profile of lipoxygenase metabolites of arachidonic acid (AA) generated by cultured rabbit tracheal epithelial (TE) cells with that produced by intact rabbit tracheal segments at baseline and following addition of exogenous AA or calcium ionophore A23187. Lipoxygenase metabolites in effluent media were resolved by high-pressure liquid chromatography and quantitated by radioimmunoassay for monohydroxyeicosanoid (HETE) and leukotriene (LT) metabolites [5-, 12-, and 15-HETE; LTB4, LTC4, LTD4]. Following incubation with exogenous AA (10 micrograms/ml), cultured TE cells generated immunoreactive products that coeluted with authentic 5-, 12-, and 15-HETE standards. 12-HETE was the predominant metabolite. Whereas the generation of HETEs by TE monolayers was dependent on addition of exogenous AA, intact tracheal segments demonstrated a baseline production of 12-HETE and lesser amounts of 5- and 15-HETE as well as unidentified metabolites with UV absorbance at 280 nm. Incubation of tracheal segments with AA resulted in augmented metabolite production. In cultured TE cells, small quantities of HETEs were present intracellularly esterified to membrane phospholipids or free in the cytosol, and significant increases in free c...Continue Reading

References

Jan 1, 1989·The American Journal of Physiology·J A GordonA A Spector
Aug 1, 1989·The American Review of Respiratory Disease·L ChurchillD Proud
Sep 1, 1989·American Journal of Respiratory Cell and Molecular Biology·J R HansbroughM J Holtzman
Sep 1, 1989·American Journal of Respiratory Cell and Molecular Biology·H Salari, M Chan-Yeung
Feb 1, 1988·The American Review of Respiratory Disease·M FujitaR E Hyatt
Jan 1, 1988·Annals of the New York Academy of Sciences·B K LamP Y Wong
Apr 1, 1985·The American Review of Respiratory Disease·T AhmedL Yerger
Jan 1, 1986·Experimental Lung Research·G L XuT Eling
Jan 1, 1988·The American Journal of Physiology·G E RevtyakW B Campbell
Jan 1, 1988·General Pharmacology·T E ElingR Danilowicz
Dec 1, 1988·Archives of Biochemistry and Biophysics·D HenkeT E Eling
Sep 25, 1986·The New England Journal of Medicine·J J MurrayJ A Oates
Jul 1, 1980·The Journal of Pharmacy and Pharmacology·M J SmithM A Bray
Oct 16, 1980·Biochemical and Biophysical Research Communications·W F StensonT J Sullivan
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S E DahlénB Dahlén
Aug 12, 1983·Biochemical and Biophysical Research Communications·M J HoltzmanE J Goetzl
Mar 1, 1984·The American Journal of Physiology·D L ColemanJ H Widdicombe
Aug 1, 1983·The Journal of Clinical Investigation·R S KentA R Whorton
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J MacloufP Borgeat

❮ Previous
Next ❯

Citations

Aug 12, 1998·American Journal of Respiratory Cell and Molecular Biology·J TamaokiA Nagai
Jan 1, 1991·Experimental Lung Research·S E Alpert, R W Walenga
May 1, 1992·Experimental Lung Research·L A Cohn, K B Adler
Nov 15, 1994·Annals of the New York Academy of Sciences·M J HoltzmanJ D Wilson
Jan 1, 1991·Toxicologic Pathology·L A Cohn, K B Adler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.