Generation of microalga Chlamydomonas reinhardtii expressing shrimp antiviral dsRNA without supplementation of antibiotics

Scientific Reports
Patai CharoonnartVanvimon Saksmerprome

Abstract

RNA interference (RNAi) is an effective way of combating shrimp viruses by using sequence-specific double-stranded (dsRNA) designed to knock down key viral genes. The aim of this study was to use microalgae expressing antiviral dsRNA as a sustainable feed supplement for shrimp offering viral protection. In this proof of concept, we engineered the chloroplast genome of the green microalga Chlamydomonas reinhardtii for the expression of a dsRNA cassette targeting a shrimp yellow head viral gene. We used a previously described chloroplast transformation approach that allows for the generation of stable, marker-free C. reinhardtii transformants without the supplementation of antibiotics. The generated dsRNA-expressing microalgal strain was then used in a shrimp feeding trial to evaluate the efficiency of the algal RNAi-based vaccine against the virus. Shrimps treated with dsRNA-expressed algal cells prior to YHV infection had 50% survival at 8 day-post infection (dpi), whereas 84.1% mortality was observed in control groups exposed to the YHV virus. RT-PCR using viral specific primers revealed a lower infection rate in dsRNA-expressing algae treated shrimp (55.6 ± 11.1%) compared to control groups (88.9 ± 11.1% and 100.0 ± 0.0%, res...Continue Reading

References

Dec 24, 2003·Trends in Biotechnology·Rosa León-BañaresEmilio Fernández
May 27, 2009·Biologicals : Journal of the International Association of Biological Standardization·Raymond SurzyckiStefan Surzycki
Aug 29, 2009·Journal of Virological Methods·Vanvimon SaksmerpromeBoonsirm Withyachumnarnkul
Feb 19, 2013·Journal of Biotechnology·Vanvimon SaksmerpromeBoonsirm Withyachumnarnkul
Mar 7, 2014·Methods in Molecular Biology·Chloe EconomouSaul Purton
Aug 20, 2014·AMB Express·Alma Lorena Almaraz-DelgadoJesús Agustín Badillo-Corona
Sep 5, 2015·The Plant Journal : for Cell and Molecular Biology·Masaki OdaharaYasuhiko Sekine
May 6, 2016·Frontiers in Plant Science·Lior DoronMichal Shapira
Apr 13, 2018·Biology·Patai CharoonnartVanvimon Saksmerprome

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Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

SPSS

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