Generation of monoclonal antibodies of desired specificity using chimeric polyomavirus-derived virus-like particles

Journal of Immunological Methods
Aurelija ZvirblieneKestutis Sasnauskas

Abstract

Foreign protein sequences presented on hamster polyomavirus (HaPyV) major capsid protein VP1-derived virus-like particles (VLPs) have been demonstrated to be highly immunogenic. The current study was aimed to evaluate VP1-derived chimeric VLPs as tools for hybridoma technology to generate monoclonal antibodies (mAbs) of desired specificity. Chimeric VLPs containing inserts of different size and origin were used as immunogens. Chimeric VLPs carrying a 9 amino acid (aa)-long cytotoxic T-cell epitope (STAPPVHNV) of human mucin 1 (MUC1) elicited a strong epitope-specific humoral immune response in mice and promoted the production of MUC1-specific mAbs. From a total of seven mAbs of IgG isotype generated against the chimeric VLPs, two mAbs were directed against the MUC1 epitope and five mAbs against the VP1-carrier. Two out of five anti-VP1 mAbs recognized epitopes located at the previously defined insertion site #2 (aa 223/224), which confirms its surface-exposed localization. Chimeric VLPs carrying a 120-aa long sequence of Puumala hantavirus (PUUV) nucleocapsid protein (NP) promoted the generation of five mAbs of IgG isotype specific to PUUV NP. All mAbs recognized the full-length NP of different PUUV strains. In contrast, no VP1...Continue Reading

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Citations

Jul 26, 2012·Archives of Virology·Mathias SchlegelBernd Köllner
May 31, 2013·Archives of Virology·Luis J MuñozDolores E López
Feb 26, 2013·Journal of Biomedical Science·Indre DalgedieneAurelija Zvirbliene
Mar 6, 2007·Journal of Immunological Methods·Nileena VelappanAndrew R M Bradbury
Jun 12, 2013·Journal of Neurochemistry·Ramune MorkunieneVilmante Borutaite
Oct 17, 2014·Journal of Molecular Recognition : JMR·Marc HovestädtWalter F M Stöcklein
Sep 4, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Erik A TeunissenEnrico Mastrobattista
Sep 1, 2012·European Journal of Microbiology & Immunology·K MesserschmidtK Heilmann
Sep 6, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Stephan DrewesRainer G Ulrich
Jun 3, 2021·Viruses·Burkhard JandrigRainer G Ulrich

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