Generation of mouse ES cell lines engineered for the forced induction of transcription factors.

Scientific Reports
Lina S Correa-CerroMinoru S H Ko

Abstract

Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.

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Citations

Jun 12, 2014·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Alexei A SharovMinoru S H Ko
Aug 1, 2015·Journal of Bioinformatics and Computational Biology·Alexei A SharovMinoru S H Ko
Aug 16, 2014·Cell·Patrick CahanJames J Collins
Apr 17, 2016·Nucleic Acids Research·Yair AaronsonEran Meshorer
Dec 29, 2013·Stem Cell Reports·Kohei YamamizuMinoru S H Ko
May 29, 2016·Biochemical and Biophysical Research Communications·Neda YahooFardin Fathi
Oct 22, 2016·Trends in Genetics : TIG·Michael R Brent
Oct 18, 2016·Stem Cell Reports·Xiaofeng ZhengGuang Hu
Apr 28, 2017·Nature Protocols·Arthur H RadleyPatrick Cahan
Jan 10, 2020·Journal of Cellular Biochemistry·Jordan C CaddyFred B Berry
Oct 22, 2016·In Vitro Cellular & Developmental Biology. Animal·Yusuke Teratani-OtaAlexei A Sharov
Jul 15, 2017·The Journal of Pathology·Erik OliemullerBeatrice A Howard
May 23, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Johannes Meisig, Nils Blüthgen
Jun 9, 2020·Frontiers in Cellular Neuroscience·Lea J FlitschOliver Brüstle
Feb 3, 2021·Nature Genetics·Riccardo M BettoGraziano Martello
Aug 1, 2021·The Journal of Biological Chemistry·Asra AlmubarakFred B Berry

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Datasets Mentioned

BETA
GSE31381

Methods Mentioned

BETA
transgenic
PCR
genotyping

Software Mentioned

NIA Array Analysis
TMEV
PAGE

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