PMID: 9530621Apr 8, 1998Paper

Generation of mutant mice with large chromosomal deletion by use of irradiated ES cells--analysis of large deletion around hprt locus of ES cell

Mammalian Genome : Official Journal of the International Mammalian Genome Society
A KushiH Sasai

Abstract

A method of generating mice from embryonic stem (ES) cells with a large chromosomal deletion produced by X-ray irradiation has been developed. Fifty-two mutant ES clones were made that carried a nested set of chromosomal deletions up to approximately 10 cM in length around the hprt locus on the X Chromosome (Chr). Germline chimeras were generated from three ES clones with deletions ranging from 200 to 700 kb. In germline male mice from two independent clones, deletions around the hprt locus yielded a runty phenotype or caused death at birth. The runty mice had approximately 1/3 the body weight and size of wild littermates and did not survive more than 3 weeks after birth. The most plausible cause of these phenotypes is defects in regions flanking the hprt locus. This method of creating mutant mice with a large chromosomal deletion is very useful for the identification and understanding of gene functions.

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Citations

Oct 26, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Wallace S H ChickYun You
Jun 14, 2005·Nature Reviews. Genetics·Benjamin T Kile, Douglas J Hilton
Apr 8, 2006·BMC Biochemistry·Luca JovinePaul M Wassarman
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F LePageR A Conlon
Jul 6, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Wen-Lin ChangHongmei Wang
Nov 2, 2004·Experimental Animals·Atsuko KushiHitoshi Sasai
Dec 23, 2006·Molecular Reproduction and Development·Michael FantRoger Bick
Jan 10, 2008·International Journal of Cancer. Journal International Du Cancer·Xue-Yuan DongWei-Feng Chen
Oct 5, 2001·Nature Reviews. Genetics·Y Yu, A Bradley
Dec 5, 2002·Physiological Genomics·Louise van der WeydenAllan Bradley
Jul 12, 2002·Environmental and Molecular Mutagenesis·Vasily N DobrovolskyRobert H Heflich
Jun 26, 2012·Molecular Reproduction and Development·Suzanne M JackmanMichael E Fant
Sep 21, 2000·Genomics·M CocchiaD Schlessinger

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