Generation of mycobacterial lipoarabinomannan-specific monoclonal antibodies and their ability to identify mycobacterium isolates.

Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi
Zhuo-Hong YanHong-Tao Zhang

Abstract

The World Health Organization has recommended commercial urine-sourced lipoarabinomannan (LAM) detection as a tool for screening HIV patients with suspected TB, but more sensitive immunodetection assays would help to identify HIV-negative TB patients. Here, we aimed to develop novel rabbit monoclonal antibodies (mAbs) against LAM for immunodetection purposes. Rabbits were immunized with cell-wall components from the Mycobacterium tuberculosis (Mtb) H37Rv strain. An immune single-chain fragment variable (scFv) phage display library was generated. The scFv mAbs to LAM were identified through ELISA screening. The light and heavy chain variable region genes from the selected clones were sequenced. Vectors containing the full-length light and heavy chains were constructed and co-expressed in 293 T cells to generate whole IgG antibodies. The performances and binding characteristics of the mAbs against purified LAM from M.tb H37Rv, multiple mycobacteria species (M.tb H37Rv, M. bovis and non-tuberculous mycobacteria (NTM) strains), and mycobacteria clinical isolates (Mtb and NTM isolates) were determined using various immunoassay methods. We obtained five rabbit mAbs against LAM, four of which had high sensitivities (100 pg/ml) and aff...Continue Reading

References

May 27, 2003·Biochimie·Jérôme NigouGermain Puzo
Jun 1, 2005·Journal of the American Society for Mass Spectrometry·Christopher J PetzoldJulie A Leary
Feb 6, 2007·American Journal of Respiratory and Critical Care Medicine·David E GriffithUNKNOWN Infectious Disease Society of America
Apr 24, 2015·PloS One·Beston HamasurStefan Svenson
May 22, 2016·Respiration; International Review of Thoracic Diseases·Nasstasja WassilewChristoph Lange
Jan 28, 2017·Microbiology Spectrum·Won-Jung Koh
Apr 4, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alok ChoudharyAbraham Pinter
Jun 14, 2018·Memórias do Instituto Oswaldo Cruz·Marcio Roberto SilvaMark Drew Crosland Guimarães
Nov 6, 2018·Frontiers in Immunology·Mathieu DondelingerMarylène S Vandevenne
Feb 19, 2019·ERJ Open Research·Margarida Correia-NevesGunilla Källenius

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Citations

May 4, 2021·Frontiers in Microbiology·Julio FloresLeslie Chavez-Galan
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust

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