Generation of new human embryonic stem cell lines with diploid and triploid karyotypes

Development, Growth & Differentiation
Hossein BaharvandAli Farrokhi

Abstract

Human pluripotent embryonic stem cells (hESC) have great promise for research into human developmental biology and the development of cell therapies for the treatment of diseases. To meet the increased demand for characterized hESC lines, we present the derivation and characterization of five hESC lines on mouse embryonic fibroblast cells. Our stem cell lines are characterized by morphology, long-term expansion, and expression profiles of a number of specific markers, including TRA-1-60, TRA-1-81, alkaline phosphatase, connexin 43, OCT-4, NANOG, CXCR4, NODAL, LEFTY2, THY-1, TDGF1, PAX6, FOXD3, SOX2, EPHA2, FGF4, TAL1, AC133 and REX-1. The pluripotency of the cell line was confirmed by spontaneous differentiation under in vitro conditions. Whereas all of the cell lines expressed all the characteristics of undifferentiated pluripotent hESC, two of the cell lines carried a triploid karyotype.

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Citations

Apr 25, 2012·Journal of Assisted Reproduction and Genetics·Xinjie ChenXiaofang Sun
Feb 24, 2010·In Vitro Cellular & Developmental Biology. Animal·Adeleh TaeiHossein Baharvand
Dec 19, 2012·Molecular Biotechnology·Faranak FattahiHossein Baharvand
Sep 18, 2008·Cloning and Stem Cells·Chanchao LorthongpanichAnthony W S Chan
Jan 5, 2011·Stem Cells and Development·Mehran Rezaei LarijaniHossein Baharvand
Sep 24, 2009·Tissue Engineering. Part a·Fabian LangenbachJörg Handschel
Mar 11, 2010·Tissue Engineering. Part a·Jörg HandschelUlrich Meyer
Jul 12, 2011·Tissue Engineering. Part a·Ebrahim ShahbaziHossein Baharvand
Oct 31, 2009·Tissue Engineering. Part C, Methods·Justyna JozefczukJames Adjaye
Jun 12, 2008·Head & Face Medicine·Jörg HandschelUlrich Meyer
Jul 14, 2011·Regenerative Medicine·Juan-Carlos Biancotti, Nissim Benvenisty
Jul 4, 2012·Indian Journal of Human Genetics·Mohammad H Kariminejad, Ardeshir Khorshidian
Nov 15, 2011·Stem Cell Reviews and Reports·Samira AsgariHossein Baharvand
Feb 22, 2012·Stem Cell Reviews and Reports·Yousof GheisariMohammad Vasei
Jun 8, 2006·Proteomics·Hossein BaharvandGhasem Hosseini Salekdeh
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May 15, 2007·Stem Cells·Hossein BaharvandGhasem Hosseini Salekdeh
Apr 9, 2013·Journal of Cellular Biochemistry·Chunyan JiangJiayin Liu
Aug 18, 2012·The Journal of Pathology·Philipp Grosse-GehlingLeoni A Kunz-Schughart
Jan 8, 2008·Differentiation; Research in Biological Diversity·Hossein BaharvandMansoureh Shahsavani
Apr 25, 2015·Journal of Biomedical Materials Research. Part a·Mohammad Hossein GhanianHossein Baharvand
Sep 1, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Majdaddin RezaeiParvaneh Nikpour

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