PMID: 9554832Apr 29, 1998Paper

Generation of oxygen radicals from iron complex of orellanine, a mushroom nephrotoxin; preliminary ESR and spin-trapping studies

Free Radical Research
D Cantin-EsnaultA Jeunet

Abstract

Orellanine, [2,2'-bipyridine]-3,3',4,4'-tetrol-1,1'-dioxide, is the toxin responsible for the lethal nephrotoxicity of some Cortinarius mushrooms. Our present ESR and spin-trapping studies of the redox properties of the system of non-illuminated orellanine, ferrous iron and dioxygen contribute to understanding the molecular mechanism of its toxicity. UV-visible spectrophotometry, cyclic voltammetry and ESR in frozen medium showed the formation of a wine-red tris complex, Fe(III)Or3. This ferric complex is easily reducible (Ep = -565 mV vs Ag/AgCl/3M KCl at pH 7), involving a one-electron reversible process. Spin-trapping using DMPO is employed to detect the generation of superoxide anion and hydroxyl radicals. The instantaneous one-electron oxidation of ferrous ions in the presence of the toxin under air is concomitant with dioxygen consumption as supported by dioxygen consumption. GSH involves the toxin and ferrous ions under air in a redox cycling process resulting in the production of glutathionyl and oxygen free radicals, observed for the first time with an iron complex of a mushroom toxin. In most cases, EDTA is not able to prevent the Fe(III)Or3 and radical formation. The ortho-dihydroxylated groups borne by the di-N-oxid...Continue Reading

References

Nov 15, 1990·Biochemical and Biophysical Research Communications·K MakinoA Murakami
Jan 1, 1990·Free Radical Biology & Medicine·D M MillerS D Aust
Jan 1, 1988·Archives of Toxicology·J M RichardD Cantin

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Citations

Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·V C DanelD Garon
Mar 10, 2000·Toxicon : Official Journal of the International Society on Toxinology·A ZhelevaZ D Zhelev
Feb 5, 2015·Hemodialysis International·Pasquale EspositoAntonio Dal Canton
Aug 24, 2013·Renal Failure·Scott-Oliver GrebeStefan Herget-Rosenthal
Feb 19, 2008·Free Radical Biology & Medicine·Ulf A NilssonBörje Haraldsson
Nov 11, 2015·Human & Experimental Toxicology·Ricardo Jorge Dinis-OliveiraFélix Carvalho
Aug 22, 2018·Toxins·Deman NajarKerstin Ebefors

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