Generation of phase II in vitro metabolites using homogenized horse liver

Drug Testing and Analysis
Jenny K Y WongTerence S M Wan

Abstract

The successful use of homogenized horse liver for the generation of phase I in vitro metabolites has been previously reported by the authors' laboratory. Prior to the use of homogenized liver, the authors' laboratory had been using mainly horse liver microsomes for carrying out equine in vitro metabolism studies. Homogenized horse liver has shown significant advantages over liver microsomes for in vitro metabolism studies as the procedures are much quicker and have higher capability for generating more in vitro metabolites. In this study, the use of homogenized liver has been extended to the generation of phase II in vitro metabolites (glucuronide and/or sulfate conjugates) using 17β-estradiol, morphine, and boldenone undecylenate as model substrates. It was observed that phase II metabolites could also be generated even without the addition of cofactors. To the authors' knowledge, this is the first report of the successful use of homogenized horse liver for the generation of phase II metabolites. It also demonstrates the ease with which both phase I and phase II metabolites can now be generated in vitro simply by using homogenized liver without the need for ultracentrifuges or tedious preparation steps.

References

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Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fan PuMalcolm D McLeod
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Mar 8, 2011·Drug Testing and Analysis·Jenny K Y WongTerence S M Wan
Feb 1, 2014·Journal of Veterinary Pharmacology and Therapeutics·H K KnychD S McKemie
Feb 4, 2014·The Veterinary Journal·Jenny K Y Wong, Terence S M Wan

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Citations

Oct 13, 2016·Drug Testing and Analysis·Christopher C Waller, Malcolm D McLeod
Jun 22, 2017·Drug Testing and Analysis·Sumudu A WeththasingheMalcolm D McLeod
Aug 28, 2020·Drug Testing and Analysis·Yat-Ming SoEmmie N M Ho
Jan 14, 2021·Drug Testing and Analysis·Fernando MoreiraMaria de Lourdes Bastos
Jan 19, 2021·Drug Testing and Analysis·Moses PhilipAbdul Khader Karakka Kal

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