Generation of phenylpropanoid pathway-derived volatiles in transgenic plants: rose alcohol acetyltransferase produces phenylethyl acetate and benzyl acetate in petunia flowers

Plant Molecular Biology
Inna GutermanAlexander Vainstein

Abstract

Esters are important contributors to the aroma of numerous flowers and fruits. Acetate esters such as geranyl acetate, phenylethyl acetate and benzyl acetate are generated as a result of the action of alcohol acetyltransferases (AATs). Numerous homologous AATs from various plants have been characterized using in-vitro assays. To study the function of rose alcohol acetyltransferase (RhAAT) in planta, we generated transgenic petunia plants expressing the rose gene under the control of a CaMV-35S promoter. Although the preferred substrate of RhAAT in vitro is geraniol, in transgenic petunia flowers, it used phenylethyl alcohol and benzyl alcohol to produce the corresponding acetate esters, not generated by control flowers. The level of benzyl alcohol emitted by the flowers of different transgenic lines was ca. three times higher than that of phenylethyl alcohol, which corresponded to the ratio between the respective products, i.e. ca. three times more benzyl acetate than phenylethyl acetate. Feeding of transgenic petunia tissues with geraniol or octanol led to the production of their respective acetates, suggesting the dependence of volatile production on substrate availability.

References

Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·N DudarevaE Pichersky
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·B M LangeR Croteau
Dec 18, 2001·Plant Physiology·A VainsteinD Weiss
Feb 5, 2003·The Plant Cell·Feng ChenJonathan Gershenzon
Feb 19, 2003·Phytochemistry·Julian C VerdonkRobert C Schuurink
Nov 25, 2003·The Plant Cell·Florence NegreNatalia Dudareva
Aug 3, 2004·Plant Physiology·Jennifer BoatrightNatalia Dudareva
Aug 25, 2004·Plant Physiology·Jules BeekwilderAsaph Aharoni
Aug 25, 2004·Plant Physiology·Natalia DudarevaJonathan Gershenzon
Jan 18, 2005·Current Opinion in Plant Biology·Natalia Dudareva, Florence Negre
Apr 5, 2005·The Plant Cell·Julian C VerdonkRobert C Schuurink
Apr 26, 2005·Plant Physiology·Beverly A UnderwoodDavid G Clark
Apr 30, 2005·Current Opinion in Plant Biology·Eyal Fridman, Eran Pichersky
May 3, 2005·Annual Review of Plant Biology·David R Gang
May 28, 2005·The Plant Journal : for Cell and Molecular Biology·Dorothea ThollEran Pichersky

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Citations

Oct 1, 2010·Biotechnology Letters·Stephen F Chandler, Filippa Brugliera
Nov 11, 2009·Journal of Chemical Ecology·Astrid KännasteAnna-Karin Borg-Karlson
Jul 28, 2011·Plant & Cell Physiology·Yu-Yun HsiaoHong-Hwa Chen
Sep 30, 2010·Plant Physiology·Ira MartonAlexander Vainstein
Jan 31, 2012·Applied and Environmental Microbiology·Frauke LüddekeJens Harder
May 22, 2007·Biochemistry. Biokhimii︠a︡·L XiangL-Q Chen
Nov 21, 2012·BMC Genomics·Annick DuboisMohammed Bendahmane
Dec 24, 2011·PloS One·Annick DuboisMohammed Bendahmane
Feb 1, 2013·Journal of Experimental Botany·Mohammed BendahmaneManuel Le Bris
Feb 19, 2013·Plant Science : an International Journal of Experimental Plant Biology·Pankaj K SharmaRajender S Sangwan
Jan 20, 2007·Trends in Biotechnology·Eran Pichersky, Natalia Dudareva
Mar 19, 2008·Plant Biotechnology Journal·Michal Moyal Ben ZviAlexander Vainstein
May 10, 2008·The Plant Journal : for Cell and Molecular Biology·Yu-Yun HsiaoHong-Hwa Chen
Mar 8, 2012·Journal of the Science of Food and Agriculture·Chia-Hung KuoChwen-Jen Shieh
Feb 7, 2013·The New Phytologist·Natalia DudarevaIan Kaplan
Jul 19, 2013·Plant, Cell & Environment·Kolby JardineChristiane Werner
Mar 26, 2014·The Plant Journal : for Cell and Molecular Biology·Edwige J F SouleyreRoss G Atkinson
Apr 9, 2008·Current Opinion in Biotechnology·Natalia Dudareva, Eran Pichersky
Oct 3, 2014·Bioscience, Biotechnology, and Biochemistry·Ruijie HaoQixiang Zhang
Mar 7, 2017·The New Phytologist·Jasmin RavidAlexander Vainstein
Dec 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana D Q MeloFrancisco A Dias Filho
Nov 26, 2016·Bioscience, Biotechnology, and Biochemistry·Sayaka KimuraTakashi Iwasaki
Feb 20, 2021·Journal of Experimental Botany·Ryan M PatrickYing Li

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