Generation of rat induced pluripotent stem cells using a plasmid vector and possible application of a keratan sulfate glycan recognizing antibody in discriminating teratoma formation phenotypes

Biological & Pharmaceutical Bulletin
Juliet O MakangaTetsuya Inazu

Abstract

Induced pluripotent stem cells (iPSCs) offer an invaluable tool for biological research and regenerative medicine. We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc and Klf4. Although all riPSC clones were generated and cultured under the same conditions, expressed hallmark pluripotency markers and differentiated successfully in vitro, the expression of a keratan sulfate glycan epitope with unique properties defined by R-10G antibody varied in the riPSC clones. In contrast, tumor rejection antigen (TRA)-1-81 epitope expression was comparable. A clone highly reactive to R-10G antibody formed teratomas in vivo consisting of cells from all three germ layers. However, clones expressing a lower level of the epitope defined by R-10G resulted in tumors with rapid growth consisting of undifferentiated cells. Additionally, riPSCs could be successfully differentiated into a neuronal lineage including glutamate neurons that responded to agonist stimulation. These observations demonstrate a glycophenotypic difference that may potentially serve as a useful probe for riPSC evaluation and to study the role of glycans in pluripotency and carcinogenesis in these cells.

References

Jul 11, 2009·Nature Biotechnology·Kyoko MiuraShinya Yamanaka
Mar 26, 2010·Methods in Molecular Biology·Wenlin Li, Sheng Ding
Jul 31, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ikuo TomiokaHideyuki Okano
Sep 28, 2010·Trends in Genetics : TIG·Howard J JacobAron M Geurts
Mar 11, 2011·Nature Reviews. Cancer·Uri Ben-David, Nissim Benvenisty
Apr 8, 2011·The Journal of Biological Chemistry·Hiroaki TatenoMakoto Asashima
Jun 19, 2012·Cell Stem Cell·Shinya Yamanaka
Jan 25, 2013·Proceedings of the National Academy of Sciences of the United States of America·Naoki FujitaniYasuro Shinohara
Aug 9, 2013·The Journal of Biological Chemistry·Ming-Gui JiangQi Zhou

❮ Previous
Next ❯

Citations

Dec 25, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Floriana Bulic-JakusNino Sincic
Jan 18, 2018·Glycobiology·Bruce Caterson, James Melrose
Mar 3, 2018·Biochemical and Biophysical Research Communications·Syouichi KatayamaTetsuya Inazu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.