Abstract
The prooxidant and apoptosis-inducing effects of Sho-saiko-to, a traditional Sino-Japanese herbal medicine and its active ingredients were analyzed. Among the components of Sho-saiko-to, wogon, the extract of Scutellaria and licorice root extract induced apoptosis of HL60 cells and increased the intracellular levels of reactive oxygen species. Lower concentrations (5 to 20 muM) of baicalein, the principal flavonoid in the Scutellaria root extract, showed induction of cell apoptosis and elevated the intracellular reactive oxygen species. However, the increase in the concentrations of baicalein rather inhibited the induction of apoptosis and the elevated levels of reactive oxygen species in cells. Induction of baicalein-mediated apoptosis was inhibited by addition of Tempol, the scavenger of reactive oxygen species. Glycyrrhetinic acid, an ingredient of licorice root extract, also induced apoptosis followed by increase in the intracellular reactive oxygen species. The effect of Sho-saiko-to on cell differentiation can be explained by the action of two ingredients, baicalein and glycyrrhetinic acid, which cause apoptosis and increase in reactive oxygen species in cells.
References
Jan 1, 1990·Methods in Enzymology·T R Breitman
Nov 1, 1990·Carcinogenesis·F FazalJ H Parish
Oct 1, 1994·Journal of Hepatology·T TakaharaK Shiraki
Sep 1, 1995·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·X LiZ Darzynkiewicz
Jun 1, 1996·General Pharmacology·J R Hoult, M Payá
Dec 23, 1999·Molecular Genetics and Metabolism·M YoshinoK Murakami
Apr 12, 2000·Journal of Gastroenterology and Hepatology·I Shimizu
Aug 18, 2001·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·R TsubouchiM Yoshino
Feb 14, 2002·Molecular Immunology·Shugo UedaJunji Yodoi
May 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Goshi ShiotaHironaka Kawasaki
Mar 17, 2004·Free Radical Biology & Medicine·Heidi LigeretDidier Morin
Mar 20, 2004·Nature Reviews. Drug Discovery·Kevin J BarnhamAshley I Bush
Sep 29, 2004·Biochimica Et Biophysica Acta·Cristina FioreAntonio Toninello
Nov 20, 2004·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Cynthia LevyKeith D Lindor
Jan 22, 2005·European Journal of Pharmacology·Nobuhiro OhtakeMakoto Inoue
May 3, 2005·Gynecologic Oncology·Kun ZhuNoriyuki Inaba
Citations
Dec 4, 2008·The American Journal of Chinese Medicine·Lindsey N JacksonB Mark Evers
Jul 16, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Ningning ZhengShuang Huang
Sep 28, 2010·European Journal of Pharmacology·Chung Soo LeeSoon Chul Myung
Aug 30, 2008·Life Sciences·Chung Soo LeeSun Joo Lee
Mar 10, 2007·Life Sciences·Chung Soo LeeEun Sook Han
Nov 28, 2013·Toxicological Research·Myoung-Seok KimJung-Hee Cho
Jul 15, 2012·Food Chemistry·Li-Li DongPan-Song Mu
Apr 1, 2008·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Yun Jeong Kim, Chung Soo Lee
Feb 23, 2007·The Journal of Pharmacology and Experimental Therapeutics·Soo Bin YimChung Soo Lee