Generation of reactive oxygen species by equine spermatozoa

American Journal of Veterinary Research
B A BallJ Baumber

Abstract

To characterize generation of reactive oxygen species (ROS) by equine spermatozoa. Multiple semen samples collected from 9 stallions. Equine spermatozoa were separated from seminal plasma on a discontinuous polyvinylpyrrolidone (PVP)-coated silica gradient and resuspended in a modified Tyrode albumin-lactate-pyruvate medium. Amount of hydrogen peroxide (H2O2) generated was assayed by use of a 1-step fluorometric assay, using 10-acetyl-3,7-dihydroxyphenoxazine as a probe for detection of H2O2 in a microplate assay format. Concentration of H2O2 was determined by use of a fluorescence microplate reader. Amount of H2O2 generated increased significantly with time and spermatozoa concentration for live and flash-frozen spermatozoa, and amount of H2O2 generated was significantly greater for flash-frozen than for live spermatozoa. Addition of the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) significantly increased generation of H2O2 by live and flash-frozen spermatozoa. Addition of a calcium ionophore also significantly increased the amount of H2O2 generated by live spermatozoa but did not have an effect on amount of H2O2 generated by flash-frozen spermatozoa. Abnormal equine spermatozoa generated significantly g...Continue Reading

References

Jul 1, 1992·Equine Veterinary Journal·S P BrinskoL Johnson
May 6, 1991·Biochimica Et Biophysica Acta·A R Cross, O T Jones
Mar 1, 1991·Biology of Reproduction·I BizeC Sharpe
Jun 1, 1988·Biology of Reproduction·J J ParrishN L First
Mar 1, 1995·Histochemistry and Cell Biology·J M Robinson, J A Badwey
Jan 1, 1995·Journal of Reproduction and Fertility·J F GriveauD Le Lannou
Dec 1, 1994·International Journal of Andrology·J F GriveauD Le Lannou
May 1, 1993·Journal of Reproduction and Fertility·R J AitkenD Buckingham
Feb 1, 1993·Free Radical Biology & Medicine·E de Lamirande, C Gagnon
Jan 1, 1995·Reproduction, Fertility, and Development·R J Aitken
Mar 1, 1995·Free Radical Biology & Medicine·E de Lamirande, C Gagnon
May 26, 1999·Journal of Reproduction and Fertility·R J Aitken

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Citations

May 25, 2005·Animal Reproduction Science·Juliana Almeida, Barry A Ball
Apr 3, 2004·Free Radical Biology & Medicine·R John AitkenEileen A McLaughlin
Jul 1, 2007·Animal : an International Journal of Animal Bioscience·P GogolM Cegła
Jul 18, 2002·International Journal of Andrology·R John Aitken, Mark A Baker
Mar 3, 2005·Reproduction, Fertility, and Development·R John Aitken
Aug 4, 2009·Molecular Human Reproduction·R J Aitken, G N De Iuliis
Oct 23, 2009·Biology of Reproduction·Megan J McCarthyStuart A Meyers
Nov 30, 2005·Reproductive Biology and Endocrinology : RB&E·Mark A Baker, R John Aitken
May 30, 2002·Biological Chemistry·Matilde Maiorino, Fulvio Ursini
Jun 7, 2005·American Journal of Veterinary Research·Julie BaumberJennifer J Linfor
Apr 4, 2006·American Journal of Veterinary Research·Khalida Sabeur, Barry A Ball
Mar 4, 2014·Animal Reproduction Science·C MurphyS Fair
Jul 31, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·K Sabeur, B A Ball
Oct 4, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Tamara Leahy, Bart M Gadella
Feb 18, 2016·BioMed Research International·Zamira Gibb, Robert J Aitken
Apr 27, 2012·Animal Reproduction Science·B Macías-GarcíaJ M Morrell
Jun 15, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Fiorenza MinerviniAngelo Visconti
Jan 12, 2010·Animal Reproduction Science·T LeahyW M C Maxwell
May 23, 2008·Theriogenology·D D Varner
Jun 22, 2015·Theriogenology·Joan E Rodríguez-Gil, Sergi Bonet
Jun 20, 2014·Equine Veterinary Journal·D D VarnerR J Aitken
Apr 23, 2016·Reproduction in Domestic Animals = Zuchthygiene·L Anel-LópezJ J Garde

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