PMID: 8600973Mar 15, 1996Paper

Generation of reactive oxygen species by the redox cycling of nitroprusside

Biochimica Et Biophysica Acta
D N Ramakrishna Rao, A I Cederbaum

Abstract

The formation of oxygen species during the redox cycling of sodium nitroprusside by rat liver microsomes and by chemical reductants was evaluated. The reduction of sodium nitroprusside by ascorbate and glutathione results in formation of the nitroprusside nitroxide radical which, on freezing at 77 K, results exclusively in the tetracyano [Fe(CN4)NO]2- and pentacyano [Fe(CN5)NO]3- forms of nitroxide radicals, respectively. The role of reducing agents on the inter-conversion of these two forms of nitroxide radical is discussed. The NADH and NADPH dependent microsomal reduction of nitroprusside results in the production of nitroprusside nitroxide radical, which in the presence of oxygen undergoes redox cycling to generate superoxide radical, and eventually hydroxyl radical is formed by a Fenton-type of reaction. Studies on the effect of several biologically or toxicologically relevant iron chelators on NADPH-dependent microsomal reduction of nitroprusside and subsequent formation of hydroxyl radical indicate that certain iron chelators such as isocitrate act as hydroxyl radical scavengers (depending on its concentration), but other chelators such as EDTA and DPTA function as good catalysts for the generation of hydroxyl radicals. ...Continue Reading

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Citations

May 25, 2002·Drug and Chemical Toxicology·R PandeyR K Hans
Aug 30, 2002·Biochemical and Biophysical Research Communications·Hideo KimuraHiroyasu Esumi
Dec 2, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Kyung-San MinEun-Cheol Kim
May 22, 2013·Oxidative Medicine and Cellular Longevity·Marisol Godínez-RubíDaniel Ortuño-Sahagún
May 13, 1999·Biochimica Et Biophysica Acta·R CammackM N Hughes
Apr 18, 2018·Clinical Hemorheology and Microcirculation·Muzaffer AkkocaEmine Demirel-Yilmaz
Apr 7, 2000·The Journal of Biological Chemistry·V BurkartH Kolb
Jan 30, 2003·Journal of the American Chemical Society·Maria WolakRudi van Eldik

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