PMID: 12757928May 22, 2003Paper

Generation of recombinant influenza virus using baculovirus delivery vector

Journal of Virological Methods
Kanokwan PoomputsaReingard Grabherr

Abstract

A recombinant baculovirus vector containing mammalian cell-active promoters and transcription terminators was used to deliver a mutated influenza NS gene into Vero cells. In addition to the influenza NS gene, the baculovirus contained a reporter gene expression cassette (Green fluorescent protein, GFP), allowing to monitor the Vero cell transduction efficiency. More than 90% of Vero cells were expressing GFP 24-48 h post transduction. After infecting baculovirus transduced cells with influenza helper virus, progeny of attenuated influenza virus carrying the recombinant NS gene could be selected. Baculovirus delivery was highly reproducible and efficient in Vero cells. This new method for influenza gene delivery could contribute to influenza virus research and vaccine development.

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Citations

Jun 21, 2012·Influenza and Other Respiratory Viruses·Othmar G Engelhardt
Apr 18, 2013·Expert Opinion on Biological Therapy·Maria Mercedes SeguraAlain Garnier
Jun 9, 2004·Virology·Christian KittelAndrej Egorov
Sep 26, 2006·Advances in Virus Research·Monique M van Oers
May 9, 2008·Gene Therapy·H P LeschS Ylä-Herttuala
Apr 26, 2012·Zeitschrift für Kinder- und Jugendpsychiatrie und Psychotherapie·Christian J BachmannFrank M Theisen

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