PMID: 11900840Mar 20, 2002Paper

Generation of recombinant vaccinia viruses expressing Puumala virus proteins and use in isolating cytotoxic T cells specific for Puumala virus

Virus Research
Masanori TerajimaFrancis A Ennis

Abstract

Puumala (PUU) virus causes a form of hemorrhagic fever with renal syndrome (HFRS), called nephropathia epidemica (NE), in Europe. HFRS is characterized by an increased capillary permeability, which we hypothesize is caused by hyperactivation of the host immune system, especially cellular immune responses. To identify cytotoxic T lymphocytes (CTLs) specific for the PUU virus from NE patients, we have made recombinant vaccinia viruses expressing PUU virus proteins, the nucleocapsid (N) and two surface glycoproteins, G1 and G2. Recombinant vaccinia viruses carrying the N or the first half of the G2 cDNA under the control of a strong synthetic promoter were made. To express G1 and the second half of the G2 proteins, however, we needed to use a T7 expression system, where the T7 RNA polymerase is produced from another recombinant vaccinia virus co-infecting the same cells. These recombinant vaccinia viruses were used to detect and clone PUU virus-specific CTLs from the peripheral blood mononuclear cells of NE patients. An HLA-A24-restricted CTL line recognizing the G2 protein was isolated and its 9-mer epitope was determined.

References

Dec 1, 1983·The Journal of Infectious Diseases·C S SchmaljohnJ M Dalrymple
Jul 9, 1999·Reviews in Medical Virology·M KanervaA Vaheri
Feb 13, 2001·Journal of Virology·A AlfadhliE Barklis

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Citations

Sep 25, 2012·Virus Genes·Masanori TerajimaAnita M Leporati
Mar 31, 2004·Microbes and Infection·Masanori TerajimaFrancis A Ennis
Sep 5, 2002·The Journal of Experimental Medicine·Heather L Van EppsFrancis A Ennis
Feb 3, 2009·DNA and Cell Biology·Sergei PopovRuth M Ruprecht
Jan 27, 2005·Viral Immunology·Piet MaesMarc Van Ranst
Dec 20, 2005·Viral Immunology·Masanori Terajima, Anita M Leporati
Jan 11, 2011·The Journal of Infectious Diseases·Heidi L SmithSharone Green
Sep 29, 2011·Virus Research·Adam MacneilChristina F Spiropoulou
Dec 20, 2008·Expert Review of Vaccines·Piet MaesMarc Van Ranst
Nov 26, 2013·The Journal of Infection·Tuisku-Tuulia KoivulaT Petteri Arstila
Mar 20, 2012·Human Vaccines & Immunotherapeutics·Chen ZhaoEnjie Luo
Jan 27, 2019·Vaccines·Federico Perdomo-CelisJuan C Zapata
Feb 23, 2020·Frontiers in Microbiology·Rongrong LiuXingan Wu
Feb 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elizabeth D KilpatrickFrancis A Ennis
Jul 10, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shibani Mitra-KaushikMasanori Terajima

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