Generation of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions

Fungal Biology
Nancy E Lozoya-PérezHéctor M Mora-Montes

Abstract

Sporotrichosis is an infection caused by members of the Sporothrix genus, and among them, Sporothrix schenckii is one of the etiological agents. Both, the disease and the causative agent have gained interest in the recent years, because of the report of epidemic outbreaks, and the description of the disease transmission from animals to human beings. Despite the relevance of S. schenckii in the clinical field, there are basic aspects of its biology poorly explored. So far, Agrobacterium tumefaciens-mediated transformation has been reported as an alternative for genetic manipulation of this fungal pathogen. Here, we report the optimization of the transformation method and used this to generate insertional mutants that express the green fluorescent protein in S. schenckii. We obtained five mutant strains that showed mitotic stability and expression of the reporter gene. The strains displayed normal cell wall composition, and a similar ability to interact ex vivo with human monocytes and monocyte-derived macrophages. Moreover, the virulence in larvae of Galleria mellonella was similar to that obtained with the wild-type control strains. These data indicate that these fluorescent mutants with normal ability to interact with the host...Continue Reading

Citations

Mar 12, 2019·Future Microbiology·José A Martínez-ÁlvarezHéctor M Mora-Montes
Oct 8, 2019·Current Protein & Peptide Science·Alma K Tamez-CastrellónHéctor M Mora-Montes
Jul 14, 2020·Applied Microbiology and Biotechnology·Yonghong ZhouNemat O Keyhani
Jun 12, 2021·Infection and Drug Resistance·Nancy E Lozoya-PérezHéctor M Mora-Montes
Jul 27, 2021·The Cell Surface·Alma K Tamez-CastrellónHéctor M Mora-Montes

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