Generation of structurally novel short carotenoids and study of their biological activity

Scientific Reports
Se H KimPyung C Lee

Abstract

Recent research interest in phytochemicals has consistently driven the efforts in the metabolic engineering field toward microbial production of various carotenoids. In spite of systematic studies, the possibility of using C30 carotenoids as biologically functional compounds has not been explored thus far. Here, we generated 13 novel structures of C30 carotenoids and one C35 carotenoid, including acyclic, monocyclic, and bicyclic structures, through directed evolution and combinatorial biosynthesis, in Escherichia coli. Measurement of radical scavenging activity of various C30 carotenoid structures revealed that acyclic C30 carotenoids showed higher radical scavenging activity than did DL-α-tocopherol. We could assume high potential biological activity of the novel structures of C30 carotenoids as well, based on the neuronal differentiation activity observed for the monocyclic C30 carotenoid 4,4'-diapotorulene on rat bone marrow mesenchymal stem cells. Our results demonstrate that a series of structurally novel carotenoids possessing biologically beneficial properties can be synthesized in E. coli.

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Citations

Sep 12, 2020·Microbiology Resource Announcements·Soon Jae KwonPyung Cheon Lee
Oct 10, 2020·Microbiology Resource Announcements·Soon Jae Kwon, Pyung Cheon Lee
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jun Ho LeePyung Cheon Lee
Apr 20, 2021·Biotechnology and Applied Biochemistry·Viviana Teresa OrlandiFabrizio Bolognese
May 28, 2021·3 Biotech·Subramanian PrathibaGurunathan Jayaraman
Jan 29, 2019·ACS Synthetic Biology·Ling LiDaisuke Umeno

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Methods Mentioned

BETA
PCR
glycosylation
NMR

Software Mentioned

SigmaPlot

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