Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus

Journal of Biotechnology
Ji-Yong ZhouJi-Gang Chen

Abstract

To seek a new delivery system of vaccine for infectious bronchitis virus (IBV), transgenic potato expressing full-length spike (S) protein of IBV was produced and its immunogenicity in chickens was investigated. One to three copies of S gene of IBV were randomly and stably inserted into potato (Solanum tuberrosum cv. Dongnong 303) genome by Agrobacterium tumefaciens-mediated transformation. Transcription and translation of S gene for IBV were confirmed by Northern blot and Western blot analyses in transgenic plantlets. Chickens immunized orally and intramuscularly with transgenic potato tubers expressing S protein generated the detectable levels of serum neutralizing antibodies and were protected against the challenge with the virulent IBV. In vitro secretion of interleukin 2 and T lymphocyte proliferation of spleen cells from the immunized chickens varied with the dose and the manner of vaccination with S protein derived from transgenic plants. The results indicated that S protein expressed in transgenic plants might be a new source for the production of Coronaviridae IBV vaccine.

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Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·Cuihong duZhijun Xie
Mar 11, 2005·Expert Opinion on Emerging Drugs·Richard M TwymanRainer Fischer
Mar 18, 2008·Veterinary Immunology and Immunopathology·Hong-Ying ChenKe Tu
Jul 25, 2007·Plant Biotechnology Journal·Denis J Murphy
Jan 7, 2015·Veterinary Research·Igor KolotilinRima Menassa
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Sep 8, 2009·FEMS Immunology and Medical Microbiology·Hong-Ying ChenShi-Min Li
Dec 17, 2015·Therapeutic Advances in Vaccines·Natsumi TakeyamaYoshikazu Yuki
Jun 12, 2018·Plant Biotechnology Journal·Emmanuel MargolinAnn E Meyers
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Jul 9, 2020·Plants·Balamurugan ShanmugarajWaranyoo Phoolcharoen

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