PMID: 6169846Sep 1, 1981Paper

Generation of transforming viruses in cultures of chicken fibroblasts infected with an avian leukosis virus

Journal of Virology
E StavnezerI Balazs

Abstract

During serial passages of an avian leukosis virus (the transformation-defective, src deletion mutant of Bratislava 77 avian sarcoma virus, designated tdB77) in chicken embryo fibroblasts, viruses which transformed chicken embryo fibroblasts in vitro emerged. Chicken embryo fibroblasts infected with these viruses (SK770 and Sk780) had a distinctive morphology, formed foci in monolayer cultures, and grew independent of anchorage in semisolid agar. Bone marrow cells were not transformed by these viruses. Another virus (SK790) with similar properties emerged during serial subcultures of chicken embryo fibroblasts after a single infection with tdB77. The 50S to RNAs isolated from these viruses contained a tdB77-sized genome (7.6 kilobases), 8.7- and 5.7-kilobase RNAs, and either a 4.1-kilobase RNA or a 4.6-kilobase RNA. These RNAs did not hybridize with cDNA's representing the src, erb, mac, and myb genes of avian acute transforming viruses. Cells transformed by any one of the Sk viruses (SK770, SK780, or SK790) synthesized two novel gag-related polyproteins having molecular weights of 110,000 (p110) and 125,000 (p125). We investigated the compositions of these proteins with monospecific antiviral protein sera. We found that p110 wa...Continue Reading

Citations

Jun 1, 1995·Microscopy Research and Technique·S RulongP Pinto da Silva
Feb 12, 1998·The Journal of Biological Chemistry·G ZhengE Stavnezer
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R DahlM J Hayman
Nov 16, 2004·Molecular and Cellular Biology·Nobuhide UekiMichael J Hayman
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alon M LevyHsing-Jien Kung
Jul 4, 2015·Journal of Cellular Biochemistry·Solange RivasKatherine Marcelain
Jun 13, 2018·Signal Transduction and Targeted Therapy·Angeles C Tecalco-CruzMarina Macías-Silva
Jan 27, 1998·Developmental Biology·L S AmaravadiR C Smith
Sep 1, 1989·Molecular and Cellular Biology·P Sutrave, S H Hughes
Jan 1, 1985·Pharmacology & Therapeutics·D R MakowskiS M Astrin
Jun 1, 1990·Molecular and Cellular Biology·P SutraveS H Hughes
Oct 1, 1984·Molecular and Cellular Biology·M G Katze, R M Krug
Sep 1, 1984·Molecular and Cellular Biology·O FasanoM Wigler
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Paul N MacDonaldChun Gu
Sep 25, 1999·Molecular Reproduction and Development·C J HuangH J Tsai
Sep 1, 1991·Journal of Virology·C ColmenaresE Stavnezer
Sep 1, 1990·Genes & Development·P SutraveS H Hughes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.