Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites

PloS One
Mo Sun KwonTeoan Kim

Abstract

The transgenic chicken has been considered as a prospective bioreactor for large-scale production of costly pharmaceutical proteins. In the present study, we report successful generation of transgenic hens that lay eggs containing a high concentration of human erythropoietin (hEPO) in the ovalbumin. Using a feline immunodeficiency virus (FIV)-based pseudotyped lentivirus vector enveloped with G glycoproteins of the vesicular stomatitis virus, the replication-defective vector virus carrying the hEPO gene under the control of the chicken ovalbumin promoter was microinjected to the subgerminal cavity of freshly laid chicken eggs (stage X). Stable germline transmission of the hEPO transgene to the G1 progeny, which were non-mosaic and hemizygous for the hEPO gene under the ovalbumin promoter, was confirmed by mating of a G0 rooster with non-transgenic hens. Quantitative analysis of hEPO in the egg whites and in the blood samples taken from G1 transgenic chickens showed 4,810 ~ 6,600 IU/ml (40.1 ~ 55.0 μg/ml) and almost no detectable concentration, respectively, indicating tightly regulated oviduct-specific expression of the hEPO transgene. In terms of biological activity, there was no difference between the recombinant hEPO contain...Continue Reading

References

Dec 14, 2002·Trends in Biotechnology·Robert Ivarie
Nov 7, 2003·Transgenic Research·Jeffrey C RappRobert Ivarie
Dec 4, 2003·Animal Reproduction Science·Heiner Niemann, Wilfried A Kues
Feb 15, 2005·Drug Discovery Today·Simon G LillicoHelen M Sang
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Jan 27, 2007·European Journal of Haematology·Wolfgang Jelkmann
Jan 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·S G LillicoH M Sang
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Apr 16, 2008·British Journal of Haematology·Wolfgang Jelkmann
Aug 5, 2009·Transgenic Research·Carlos Alberto PennoMasamichi Kamihira

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Citations

Mar 10, 2020·Cellular Reprogramming·Chunxia XiongXiong Xiao
Oct 21, 2021·Biology of Reproduction·Nima DehdilaniHesam Dehghani

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Datasets Mentioned

BETA
AC159826.1

Methods Mentioned

BETA
transgenic
glycosylation
transgenics
transfection
PCR
enzyme-linked immunosorbent assays
FCS
electrophoresis
ELISA

Software Mentioned

Statistical Analysis System ( SAS )

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