Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase

Prostate International
Mayuko KanayamaAtsu Aiba

Abstract

Genetically engineered mouse models are useful tools to decipher molecular mechanisms of diseases. As for prostates, a rat probasin promoter has been widely used to drive prostate-specific gene expression. To optimize its codon usage to that of mammals, we used codon-improved Cre recombinase (iCre) for prostate-specific Cre-loxP recombination. We generated transgenic mice that express iCre driven by conventional probasin promoter in a prostate-specific manner (PB-iCre). Linearized PB-iCre transgene deoxyribonucleic acids (DNAs) were microinjected into pronuclei of fertilized mouse embryos. The integration of the transgene was confirmed by Southern blot analysis. A line of transgenic mice expressing a sufficient amount of iCre mRNA in its prostate was selected. To test recombinase activity of PB-iCre in vivo, its offspring was crossbred with Ptenflox/flox mice in which murine prostate adenocarcinoma is reported to occur upon excision of loxP-flanked regions. Eight founder animals were obtained, all of which showed germ line integration of PB-iCre transgene by Southern blot analysis. Among them, the prostate from only one line (line 58) expressed a sufficient amount of iCre mRNA. This line was crossbred with Ptenflox/flox mice to...Continue Reading

Citations

Mar 7, 2020·Science Translational Medicine·Mitchell G LawrenceGail P Risbridger

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Methods Mentioned

BETA
gene knockout
transgenic
PCR

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