PMID: 8590808Dec 1, 1995Paper

Generation of truncated forms of the NG2 proteoglycan by cell surface proteolysis

Molecular Biology of the Cell
A NishiyamaW B Stallcup

Abstract

NG2 is a chondroitin sulfate proteoglycan that is expressed on dividing progenitor cells of several lineages including glia, muscle, and cartilage. It is an integral membrane proteoglycan with a core glycoprotein of 300 kDa. In the present study we have characterized three molecular forms of the NG2 core protein expressed by different cell lines. Many cell lines that express the full length 300-kDa NG2 core protein also release a 290-kDa form into the medium. This species lacks the cytoplasmic domain but contains almost the entire ectodomain. Two core protein species, the intact 300-kDa form and a truncated 275-kDa form, are expressed at the surface of an NG2-transfected cell line U251NG52. The 275-kDa species lacks the cytoplasmic domain and at least 64 amino acids of the ectodomain. Mild trypsinization of B49 cells also generates the 275-kDa species, suggesting that this component is produced by proteolysis of the 300-kDa form. Conversion of the 300-kDa species to the 275-kDa form in U251NG52 cells is stimulated by reagents such as phorbol esters, which activate protein kinase C. Phorbol esters are also known to induce expression of metalloproteinases such as collagenase and stromelysin, which could be responsible for cleavag...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·M BernfieldE J Lose
Jul 1, 1991·The Journal of Cell Biology·A NishiyamaW B Stallcup
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A Pandiella, J Massagué
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J D BuxbaumP Greengard
Apr 1, 1990·Trends in Genetics : TIG·L M Matrisian
Dec 1, 1990·The Journal of Cell Biology·W B StallcupP Healy
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S M FrischZ Werb
Aug 15, 1988·Biochemical and Biophysical Research Communications·M BallinU P Thorgeirsson
May 17, 1974·Nature·D SchubertB L Brandt
Nov 1, 1972·The Journal of Cell Biology·A L Hubbard, Z A Cohn
May 16, 1984·Biochemical and Biophysical Research Communications·A Rodriguez-Pena, E Rozengurt
Apr 15, 1981·Developmental Biology·S S WilsonW B Stallcup
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·W B StallcupJ Levine
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A SprecherD C Foster
Dec 1, 1993·Journal of Cellular Biochemistry·A Mauviel
Nov 1, 1993·Molecular Biology of the Cell·A Nishiyama, W B Stallcup

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Citations

Sep 1, 2000·Journal of Cellular Biochemistry·D S BarrittW B Stallcup
Sep 14, 1999·Brain Research Bulletin·J W Fawcett, R A Asher
Oct 29, 2002·Cellular Signalling·Mousumi MajumdarWilliam B Stallcup
Dec 11, 2003·Molecular and Cellular Neurosciences·Daniel A MorgensternJames W Fawcett
Sep 30, 2009·Neuron Glia Biology·Susan M Staugaitis, Bruce D Trapp
Dec 20, 2008·Nature Reviews. Neuroscience·Akiko NishiyamaXiaoqin Zhu
Apr 25, 2006·Journal of Neurotrauma·Nicole Klapka, Hans Werner Müller
Sep 20, 2005·Molecular and Cellular Neurosciences·Stefania PetriniEnrico Bertini
Aug 29, 2014·DNA and Cell Biology·Nam E JooYvonne L Kapila
Feb 5, 2011·Molecular and Cellular Neurosciences·Robert A HillAkiko Nishiyama
Aug 10, 2007·Biochemical and Biophysical Research Communications·Jing XiongYuxi Feng
Jan 5, 2006·Brain Pathology·Dongyan WangCandece L Gladson
Nov 11, 2003·Journal of Neurobiology·Christie D FowlerZuoxin Wang
Oct 17, 2008·Genesis : the Journal of Genetics and Development·Khalad KarramJacqueline Trotter
Feb 20, 2013·The FEBS Journal·Megan S Lord, John M Whitelock
Dec 21, 2013·Journal of Cellular Physiology·Francesca SardonePatrizia Sabatelli
Nov 13, 2009·Journal of Neuroscience Research·Makoto HoriuchiTakayuki Itoh
Jun 24, 2015·Brain Research·Dominik Sakry, Jacqueline Trotter
Mar 6, 2009·Cell Adhesion & Migration·William B Stallcup, Feng-Ju Huang
Sep 5, 2002·Journal of Cellular Biochemistry·Emmanuelle TilletWilliam B Stallcup
Apr 4, 2002·Biological Chemistry·Persio Dello Sbarba, Elisabetta Rovida

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