Generation of virus-resistant potato plants by RNA genome targeting

Plant Biotechnology Journal
Xiaohui ZhanJiang Zhang

Abstract

CRISPR/Cas systems provide bacteria and archaea with molecular immunity against invading phages and foreign plasmids. The class 2 type VI CRISPR/Cas effector Cas13a is an RNA-targeting CRISPR effector that provides protection against RNA phages. Here we report the repurposing of CRISPR/Cas13a to protect potato plants from a eukaryotic virus, Potato virus Y (PVY). Transgenic potato lines expressing Cas13a/sgRNA (small guide RNA) constructs showed suppressed PVY accumulation and disease symptoms. The levels of viral resistance correlated with the expression levels of the Cas13a/sgRNA construct in the plants. Our data further demonstrate that appropriately designed sgRNAs can specifically interfere with multiple PVY strains, while having no effect on unrelated viruses such as PVA or Potato virus S. Our findings provide a novel and highly efficient strategy for engineering crops with resistances to viral diseases.

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Citations

Aug 23, 2019·Phytopathology·Natalia O KalininaMichael E Taliansky
Oct 17, 2019·Plant Biotechnology Journal·Yaling ZhaoTong Zhang
Dec 4, 2019·Genome Biology·Ahmed MahasMagdy Mahfouz
Jul 28, 2020·Viruses·Kiran R GadhaveRajagopalbabu Srinivasan
Jan 13, 2021·Current Opinion in Plant Biology·Xueru LiuXin Li
Jan 23, 2021·Viruses·Carla Mr VarandaPatrick Materatski
Nov 17, 2020·Frontiers in Microbiology·Yongsen CaoFangfang Li
Jan 7, 2021·Plants·Michael TalianskyAndrew J Love
Feb 16, 2021·Frontiers in Microbiology·Muhammad Shafiq ShahidAbdullah M Al-Sadi
Feb 16, 2021·Frontiers in Microbiology·Leena TripathiP Lava Kumar
Jun 9, 2020·Current Opinion in Virology·Douglas Euan PyottAttila Molnar
Apr 27, 2021·Plant Communications·Shengchun LiJiang Zhang
May 13, 2021·Plant Cell Reports·Sukumar BiswasJianxin Shi
Jun 19, 2021·Transgenic Research·Cláudia RatoPaula Rodrigues Oblessuc
Jul 22, 2021·Journal of Virology·Qing WangQiwei Qin
Jan 19, 2022·Horticulture Research·Bolei JiaoYan Xu

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Datasets Mentioned

BETA
AY166867
AY745491
KU896946
KF977085

Methods Mentioned

BETA
transgenic
ELISA
PCR

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