Generation or large numbers of immature and mature dendritic cells from rat bone marrow cultures

European Journal of Immunology
M TalmorR M Steinman

Abstract

We have defined conditions for generating large numbers of dendritic cells (DC) in marrow cultures from 10-12-week-old ACI or WF rats. The combination of granulocyte-macrophage colony-stimulating factor (GM-CSF) and TNF-alpha, known to induce DC from human CD34+ progenitors, was not effective with rat. In contrast, GM-CSF plus IL-4 generated DC in high yield, corresponding to 30-40% of the initial number of plated marrow cells. The DC proliferated in distinctive aggregates, in which most cells had an immature phenotype marked by undetectable surface B7 and high levels of MHC class II products within intracellular lysosomes. When dislodged and dispersed, the aggregates gave rise to mature stellate DC with abundant surface MHC class II and B7, sparse MHC class II- lysosomes, and strong T cell-stimulating capacity. Therefore, rat marrow progenitors can generate large numbers of immature DC, with abundant intracellular MHC class II compartments, and potent, stimulatory, mature DC.

References

May 1, 1992·The Journal of Experimental Medicine·K InabaG Schuler
Jan 1, 1991·Annual Review of Immunology·M A Moore
Aug 1, 1989·The Journal of Experimental Medicine·J H JansenH C Kluin-Nelemans
Apr 1, 1986·The Journal of Experimental Medicine·W E Bowers, M R Berkowitz
Jun 1, 1985·The Journal of Cell Biology·V LewisI Mellman
Jul 1, 1982·The Journal of Experimental Medicine·W E KlinkertW E Bowers
Jul 1, 1994·The Journal of Experimental Medicine·N RomaniG Schuler

❮ Previous
Next ❯

Citations

Sep 25, 2002·Journal of Neuroscience Research·Hong Yan LiuHalina Offner
Oct 18, 2006·Cell and Tissue Research·Tomoatsu KanekoHideaki Suda
Jun 2, 2006·Apoptosis : an International Journal on Programmed Cell Death·Nicolas LarmonierBernard Bonnotte
Jun 20, 2002·Veterinary Immunology and Immunopathology·Anna VecchioneAnne Hamblin
Dec 20, 2002·Nature·Lisa M Coussens, Zena Werb
Jun 6, 2002·Immunology·Kenji HiromatsuSteven A Porcelli
Jun 9, 2009·Transplantation·Henrieta FazekasovaVincenzo Mirenda
Oct 20, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·B GoswamiA Sharma
Apr 26, 2008·Photochemistry and Photobiology·Brandon W SurEdward L Nelson
Mar 12, 2008·World Journal of Gastroenterology : WJG·Gao-Feng LuYuan-Ming Qi
Jan 9, 2003·Expert Opinion on Biological Therapy·P J WysockiA Mackiewicz
Apr 30, 2003·Japanese Journal of Cancer Research : Gann·Motoi TakeuchiChie Furihata
Sep 1, 2015·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·L HanY Cheng
Oct 26, 2002·The Journal of Veterinary Medical Science·Levi H C MakalaHideyuki Nagasawa
Jan 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Hélène PêcheMaria Cristina Cuturi
Feb 20, 2010·Veterinary Research·Meret Elisabeth Ricklin GutzwillerArtur Summerfield
Mar 22, 2013·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Gutian ZhangZeyu Sun
Sep 29, 2009·Journal of Neurochemistry·Maya Koronyo-HamaouiMichal Schwartz
Sep 5, 2001·Clinical and Experimental Immunology·B CatchpoleA S Hamblin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.