Generic technique to generate large branched DNA complexes

Biomacromolecules
Paul ToschA Giles Davies

Abstract

The inherent self-recognition properties of DNA have led to its use as a scaffold for various nanotechnology self-assembly applications, with macromolecular complexes, metallic and semiconducting nanoparticles, proteins, inter alia, being assembled onto a designed DNA scaffold. Such structures may typically comprise a number of DNA molecules organized into macromolecules. Many studies have used synthetic methods to produce the constituent DNA molecules, but this typically constrains the molecules to be no longer than around 100 base pairs (30 nm). However, applications that require larger self-assembling DNA complexes, several tens of nanometers or more, need to be generated by other techniques. Here, we present a generic technique to generate large linear, branched, and/or circular DNA macromolecular complexes. The effectiveness of this technique is demonstrated here by the use of Lambda Bacteriophage DNA as a template to generate single- and double-branched DNA structures approximately 120 nm in size.

References

Dec 20, 1987·Journal of Molecular Biology·J P Cooper, P J Hagerman
Sep 25, 1993·Nucleic Acids Research·J B WelchD M Lilley
Jul 13, 1993·Biochemistry·M ZhongN R Kallenbach
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·N C Seeman
Aug 24, 1999·Nucleic Acids Research·M S ShchepinovE M Southern
Jul 6, 2002·Science·Kinneret KerenErez Braun
Feb 22, 2003·Science·Kentaro TanakaMitsuhiko Shionoya

❮ Previous
Next ❯

Citations

Oct 13, 2006·Organic & Biomolecular Chemistry·Ye TianChengde Mao
Jun 3, 2011·Chemistry : a European Journal·Chunyan WangXiaogang Qu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

AFM in situ DNA

AFM in situ DNA describes in situ analysis (or study) of DNA using atomic force microscopy. Discover the latest research on AFM in situ DNA here.

Related Papers

Journal of the American Chemical Society
Hidenobu NakaoToshio Ohtani
Langmuir : the ACS Journal of Surfaces and Colloids
Yukiko MatsuzawaKenichi Yoshikawa
Journal of the American Chemical Society
Ozgül PersilNicholas V Hud
Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Aiguo WuErkang Wang
© 2021 Meta ULC. All rights reserved