PMID: 7536734Apr 1, 1995Paper

Genes encoded on a cyanobacterial plasmid are transcriptionally regulated by sulfur availability and CysR

Journal of Bacteriology
M L Nicholson, D E Laudenbach

Abstract

A cyanobacterial sulfur-regulated gene (cysR), which encodes a protein with similarity to the Crp family of prokaryotic regulatory proteins, has recently been isolated and characterized. Polyacrylamide gel electrophoresis of periplasmic protein extracts reveals that a cysR mutant fails to synthesize a 36-kDa polypeptide that is normally induced in wild-type cells that have been grown under sulfur-deficient conditions. The amino-terminal sequence of this protein was obtained, and a synthetic oligonucleotide was used to isolated a clone containing a 1.9-kb NruI-KpnI fragment from a Synechococcus sp. strain PCC 7942 genomic library. RNA blot analysis indicates that this fragment encodes a transcript that is detectable in wild-type but not cysR mutant cells that have been starved for sulfur. DNA blot analysis revealed that the 1.9-kb NruI-KpnI fragment is contained within the Ba4 BamHI fragment of the endogenous 50-kb plasmid pANL. RNA blot studies indicate that the accumulation of a large number of pANL transcripts is regulated by sulfur levels and CysR. DNA sequence analysis confirmed that the gene encoding the sulfur-regulated 36-kDa periplasmic protein is encoded on the Ba4 fragment of pANL. The sequence of the 36-kDa protein d...Continue Reading

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Citations

Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·R Schwarz, A R Grossman
May 12, 2001·FEMS Microbiology Reviews·C CervantesR Moreno-Sánchez
Nov 8, 2007·The FEBS Journal·César Díaz-PérezHéctor Riveros-Rosas
Oct 16, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Martha I Ramírez-DíazCarlos Cervantes
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Esther Aguilar-BarajasCarlos Cervantes
Jan 1, 1996·Annual Review of Microbiology·S Silver, L T Phung
Nov 20, 2016·FEBS Letters·Giulia MoriRiccardo Percudani
Feb 11, 2014·Journal of Bacteriology·David EncinasFernando de la Cruz
Jun 28, 2003·FEMS Microbiology Reviews·Dietrich H Nies
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Katherine A BorkovichRobert Pratt
Feb 17, 1998·Applied and Environmental Microbiology·N PeitzschD H Nies

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