Genes encoding phospholipases A2 mediate insect nodulation reactions to bacterial challenge

Journal of Insect Physiology
Sony ShresthaYonggyun Kim

Abstract

We propose that expression of four genes encoding secretory phospholipases A(2) (sPLA(2)) mediates insect nodulation responses to bacterial infection. Nodulation is the quantitatively predominant cellular defense reaction to bacterial infection. This reaction is mediated by eicosanoids, the biosynthesis of which depends on PLA(2)-catalyzed hydrolysis of arachidonic acid (AA) from cellular phospholipids. Injecting late instar larvae of the red flour beetle, Tribolium castaneum, with the bacterium, Escherichia coli, stimulated nodulation reactions and sPLA(2) activity in time- and dose-related manners. Nodulation was inhibited by pharmaceutical inhibitors of enzymes involved in eicosanoid biosynthesis, and the inhibition was rescued by AA. We cloned five genes encoding sPLA(2) and expressed them in E. coli cells to demonstrate these genes encode catalytically active sPLA(2)s. The recombinant sPLA(2)s were inhibited by sPLA(2) inhibitors. Injecting larvae with double-stranded RNAs specific to each of the five genes led to reduced expression of the corresponding sPLA(2) genes and to reduced nodulation reactions to bacterial infections for four of the five genes. The reduced nodulation was rescued by AA, indicating that expression o...Continue Reading

References

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Citations

Dec 18, 2013·Developmental and Comparative Immunology·Chuan QiaoXu-Sheng Liu
Mar 13, 2014·Journal of Insect Physiology·Rahul Hepat, Yonggyun Kim
Oct 3, 2015·Insect Biochemistry and Molecular Biology·Youngjin ParkYonggyun Kim
May 10, 2012·Journal of Invertebrate Pathology·Jung-A Park, Yonggyun Kim
Jul 18, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nina SchlotzDominik Martin-Creuzburg
Mar 21, 2020·Archives of Insect Biochemistry and Physiology·Miltan Chandra Roy, Yonggyun Kim
Jul 31, 2020·Parasites & Vectors·Wellington Junior da SilvaOnilda Santos da Silva
Feb 23, 2019·Frontiers in Physiology·David Stanley, Yonggyun Kim
Aug 15, 2018·Developmental and Comparative Immunology·Mohammad VatanparastYonggyun Kim
Feb 19, 2019·Developmental and Comparative Immunology·Mohammad VatanparastYonggyun Kim

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