Genes Encoding Proteolytic Enzymes Fungalysin and Subtilisin in Dermatophytes of Human and Animal Origin: A Comparative Study

Mycopathologia
Engin KaplanPietro Nenoff

Abstract

Dermatophytes are among the most successful fungal pathogens in humans, but their virulence mechanisms have not yet been fully characterized. Dermatophytic fungi secrete proteases in vivo, which are responsible for fungal colonization and degradation of the keratinized tissue during infection. In the present study, we used PCR to investigate the presence of genes encoding fungalysins (MEP) and subtilisins (SUB) in three dermatophyte species whose incidence is increasing in Europe: the anthropophilic Trichophyton rubrum (n = 58), zoophilic Microsporum canis (n = 33), and Trichophyton benhamiae (n = 6). MEP2 and SUB4 genes were significantly correlated with T. rubrum; MEP3 and SUB1 were mostly frequently harbored by M. canis; and MEP1, 2, and 4 and SUB3-7 were most frequently harbored by T. benhamiae isolates (p < 0.05). Furthermore, MEP1-5 and SUB1-3 genes were significantly more prevalent among human clinical isolates of M. canis (n = 17) than among asymptomatic cat isolates of M. canis (n = 16; p < 0.05). Unidentified MEP and/or SUB genes in some isolates in the current study may suggest that other gene repertoires may be involved in the degradation of keratin. The presented analysis of the incidence of MEP and SUB virulence g...Continue Reading

References

Sep 14, 2002·Infection and Immunity·Frédéric BroutaBernard Mignon
Oct 31, 2002·The Journal of Investigative Dermatology·Frédéric DescampsBernard Mignon
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Jun 12, 2016·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·J BraschP Nenoff
Oct 27, 2016·Mycopathologia·Ping Zhan, Weida Liu
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Jan 24, 2018·Medical Mycology·Derry K Mercer, Colin S Stewart
Mar 15, 2018·Medical Mycology·Yvonne GräserSybren de Hoog

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Citations

Feb 1, 2020·Mycopathologia·Vishnu Chaturvedi, G Sybren de Hoog

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Datasets Mentioned

BETA
AY437856.1
AY437857.1
AY437852.1
KU257463.1
KU257464.1

Methods Mentioned

BETA
PCRs
PCR

Software Mentioned

Primer
MegaBlast
BLAST
SPSS

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