Genes for D-arabinitol and ribitol catabolism from Klebsiella pneumoniae.

Microbiology
H HeuelJ W Lengeler

Abstract

The enzymes for catabolism of the pentitols D-arabinitol (Dal) and ribitol (Rbt) and the corresponding genes from Klebsiella pneumoniae (dal and rbt) and Escherichia coli (atl and rtl) have been used intensively in experimental evolutionary studies. Four dal and four rbt genes from the chromosome of K. pneumoniae 1033-5P14 were cloned and sequenced. These genes are clustered in two adjacent but divergently transcribed operons and separated by two convergently transcribed repressor genes, dalR and rbtR. Each operon encodes an NAD-dependent pentose dehydrogenase (dalD and rbtD), and ATP-dependent pentulose kinase (dalK and rbtK) and a pentose-specific ion symporter (dalT and rbtT). Although the biochemical reactions which they catalyse are highly similar, the enzymes showed interesting deviations. Thus, DalR (313 aa) and RbtR (270 aa) belong to different repressor families, and DalD (455 aa) and RbtD (248 aa), which are active as a monomer or as tetramers, respectively, belong to different dehydrogenase families. Of the two kinases (19.3% identity), DalK (487 aa) belongs to the subfamily of short D-xylulokinases and RbtK (D-ribulokinase; 535 aa) to the subfamily of long kinases. The repressor, dehydrogenase and kinase genes did n...Continue Reading

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Citations

Jun 10, 2011·Applied Microbiology and Biotechnology·Xue PengHideaki Yukawa
Sep 10, 2005·Plant Cell Reports·P R LaFayetteW A Parrott
Feb 13, 2001·FEMS Microbiology Letters·S Otte, J W Lengeler
Dec 20, 2011·Journal of Bacteriology·Tanja LasloBernhard J Eikmanns
Dec 30, 1999·Journal of Bacteriology·M J YebraG Pérez-Martínez
Aug 11, 2012·Bioscience, Biotechnology, and Biochemistry·Wichai SoempholHirohide Toyama
Jan 15, 2014·Applied Microbiology and Biotechnology·Hairong ChengZixin Deng
Nov 13, 2008·Journal of Bioscience and Bioengineering·Yoshikiyo Sakakibara, Badal C Saha
Jun 25, 2016·Applied Microbiology and Biotechnology·Nageena Zahid, Uwe Deppenmeier
Dec 3, 2016·The Journal of Biological Chemistry·Charandeep SinghCarole L Linster

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